b-Boswellic Acid/β-BOSWELLIC ACID/(3R,4R,4aR,6aR,6bS,8aR,11R,12S,12aR,14bR)-3-Hydroxy-4,6a,6b,8a,11,12,14b-heptamethyl-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydro-4-picenecarboxylic acid/Urs-12-en-24-oic acid, 3-hydroxy-, (3α,9ξ)-/(3a,4b)-3-Hydroxyurs-12-en-23-oic Acid/(3α,9ξ)-3-Hydroxyurs-12-en-24-oic acid
Methanol; Ethyl Acetate
556.0±50.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
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Physiological studies confirm improvement of memory by Olibanum, a resin from Boswellia species, while little is known about the molecular mechanism by which it affects memory performance. Two master transcription factors, CREB-1 and CREB-2, regulate downstream memory-related genes expression, leading to the long-term memory potentiation. This study addresses the effects of Beta-boswellic acid (β-BA), the main ingredient of Olibanum, and ethanolic extract of the resin from Boswellia serrata on the expression of CREB-1 and CREB-2 genes in B65 cell line. B65 cells were treated with β-BA or ethanolic extract of Olibanum in different doses and time intervals and the cell viability/toxicity was measured by MTT assay. Total RNA was extracted from the treated and untreated control cells and cDNA was synthesized. The expression levels of CREB-1 and CREB-2 genes were quantified by Real-time PCR. MTT assays revealed 50% inhibitory concentrations of 42.05, 29.63, and 21.78 μg/mL for ethanolic extract of Olibanum and 89.54, 44.05, and 21.12 µM for β-BA at 24, 48, and 72 h time intervals respectively. Both β-BA and ethanolic extract of Olibanum altered CREB-1 and CREB-2 gene expression levels in time-dependent but not in dose-dependent way. However, β-BA showed stronger and more stable effects. The expression levels of the both genes followed an alternate upregulation and downregulation pattern, but in opposite directions, in response to the both solutions with the progress of time. These results suggest that Olibanum possibly improves memory performance, at least partially, by regulating the levels of CREB-1 and CREB-2 transcription factors via positive/negative-feedback loops.
Beta-boswellic acid; CREB-1; CREB-2; Memory; Olibanum
Beta-Boswellic Acid and Ethanolic Extract of Olibanum Regulating the Expression Levels of CREB-1 and CREB-2 Genes.
Jebelli A1, Khalaj-Kondori M1, Bonyadi M1, Hosseinpour Feizi MA1, Rahmati-Yamchi M2,3.
Herpes Simplex Virus (HSV), a highly contagious pathogen, is responsible for causing lifelong oral to genital infection in human. Boswellia serrata oleo-gum-resin possesses a strong traditional background of treating diverse skin ailments including infection but its effect on HSV-1 has not been examined yet.
To exploit its potential, we aimed to explore the antiviral activity of methanol extract of B. serrata oleo-gum-resin (BSE) and one of its major constituent β-boswellic acid (BA) against HSV-1 along with the underlying mechanism of action involved.
BSE was subjected to RP-HPLC analysis to quantify the active constituent. Cytotoxicity (CC50) and antiviral activity were evaluated by MTT and plaque reduction assay, followed by the determination of median effective concentration (EC50). The mode of antiviral activity was assessed by time-of-addition assay and confirmed by reverse transcriptase-PCR (RT-PCR). Further, the expressions of various cytokines were measured by RT-PCR, while the proteins by Western blot.
BSE and BA potently inhibited wild-type and a clinical isolate of HSV-1 (EC50 5.2-6.2 and 12.1-14.63 μg/ml), with nearly-complete inhibition (EC99) at 10 and 30 μg/ml, respectively. The inhibitory effect was significant at 1 h post-infection and effective up to 4 h. Based on target analysis we examined the inhibition of NF-κB, essential for virus replication, and observed significant down-regulation of NF-κB, and p38 MAP-kinase activation, with reduced expression of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β and IL-6, involved in scheming NF-κB signaling.
Thus, our results support the ethnomedicinal use of BSE in skin infection by inhibiting HSV-1 through the modulation of NF-κB and p38 MAPK pathway.
Copyright © 2018 Elsevier GmbH. All rights reserved.
ATCC; American type culture collection; BA; BSE; Boswellia serrata; Boswellia serrata oleo-gum-resin extract; CC(50); DMEM; DMSO; Dimethyl sulfoxide; Dulbecco's modified minimum essential medium; EC(50); FBS; Fetal bovine serum; HBA; HBD; HSV; Herpes simplex virus; Hydrogen-bond acceptors; Hydrogen-bond donors; IL; Interleukin; MAPK; MW; Median cytotoxic concentration; Median effective concentration; Mitogen activated protein kinase; Molecular weight; NF-κB; Nuclear factor kappa-light-chain-enhancer of activated B cells; PRA; Plaque reduction assay; RP-HPLC; RT-PCR; Reverse phase high performance liquid chromatography; Reverse transcriptase polymerase chain reaction; TNF; Tumor necrosis factor; UNPD; United natural product database; p38; β-boswellic acid
Boswellia serrata oleo-gum-resin and β-boswellic acid inhibits HSV-1 infection in vitro through modulation of NF-кB and p38 MAP kinase signaling.
Goswami D1, Mahapatra AD2, Banerjee S3, Kar A3, Ojha D2, Mukherjee PK3, Chattopadhyay D4.
2018 Dec 1