We Offer Worldwide Shipping
Login Wishlist

2′-dI

$96

  • Brand : BIOFRON

  • Catalogue Number : BD-D1126

  • Specification : HPLC≥98%

  • CAS number : 890-38-0

  • Formula : C10H12N4O4

  • Molecular Weight : 252.23

  • PUBCHEM ID : 135398593

  • Volume : 20mg

Available on backorder

Quantity
Checkout Bulk Order?

Catalogue Number

BD-D1126

Analysis Method

Specification

HPLC≥98%

Storage

2-8°C

Molecular Weight

252.23

Appearance

Botanical Source

Structure Type

Category

SMILES

C1C(C(OC1N2C=NC3=C2N=CNC3=O)CO)O

Synonyms

INOSINE, 2'-DEOXY-/Inosine,2'-deoxy/9H-Purin-6-ol, 9-(2-deoxy-β-D-erythro-pentofuranosyl)-/2'-Deoxyinosine/9-(2-deoxy-b-D-erythro-pentofuranosyl)-Hypoxanthine/9-(2-deoxy-β-D-erythro-pentofuranosyl)-Hypoxanthine/9-(2-Deoxy-b-D-erythro-pentofuranosyl)-1,9-dihydro-6H-purin-6-one/9-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-9H-purin-6-ol/Deoxyinosine/(-)-2'-Deoxyinosine/d-Ino/2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE TRISODIUM SALT,DGTP

IUPAC Name

9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one

Applications

Density

1.9±0.1 g/cm3

Solubility

Flash Point

329.1±34.3 °C

Boiling Point

620.6±65.0 °C at 760 mmHg

Melting Point

250 °C

InChl

InChI=1S/C36H36O17/c1-15-26(42)30(46)34(53-35-31(47)29(45)27(43)23(51-35)14-48-24(41)11-4-16-2-7-18(37)8-3-16)36(49-15)52-33-28(44)25-21(40)12-20(39)13-22(25)50-32(33)17-5-9-19(38)10-6-17/h2-13,15,23,26-27,29-31,34-40,42-43,45-47H,14H2,1H3/b11-4+/t15-,23+,26-,27+,29-,30+,31+,34+,35-,36-/m0/s1

InChl Key

VGONTNSXDCQUGY-RRKCRQDMSA-N

WGK Germany

RID/ADR

HS Code Reference

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:890-38-0) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

No Technical Documents Available For This Product.

PMID

31123731

Abstract

PAGE and UV melting analysis revealed that longer LNA-based splice-switching oligonucleotides (SSOs) formed secondary structures by themselves, reducing their effective concentration. To avoid such secondary structure formation, we introduced 7-deaza-2′-deoxyguanosine or 2′-deoxyinosine into the SSOs. These modified SSOs, with fewer secondary structures, showed higher exon skipping activities.

Title

Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides.

Author

Shimo T 1, Tachibana K , Kawawaki Y , Watahiki Y , Ishigaki T , Nakatsuji Y , Hara T , Kawakami J , Obika S .

Publish date

2019 Jun 11

PMID

29522920

Abstract

Deamination of adenine can occur spontaneously under physiological conditions to generate the highly mutagenic lesion, deoxyinosine (hypoxanthine deoxyribonucleotide, dI). In DNA, dI preferably pairs with cytosine rather than thymine and results in A:T to G:C transition mutations after DNA replication. The deamination of adenine is enhanced by ROS from exposure of DNA to ionizing radiation, UV light, nitrous acid, or heat. In Escherichia coli, dI repair is initiated by endonuclease V (endo V; nfi gene product) nicking but a complete repair mechanism has yet to be elucidated. Using in vitro minimum component reconstitution assays, we previously showed that endo V, DNA polymerase I (pol I), and E. coli DNA ligase were sufficient to repair this dI lesions efficiently and that the 3′-5′ exonuclease of pol I is essential. Here we employed a phagemid-based T-I substrate mimicking adenine deamination product to demonstrate pol I proofreading exonuclease is required by the endo V repair pathway both in vitro and in vivo. In vivo we found that the repair level of an nfi mutant (11%) was almost 8-fold lower than the wild type (87%). while the polA-D424A strain, a pol I mutant defective in 3′-5′ exonuclease, showed a high repair level similar to wild type (both more than 80%). Using additional C-C mismatch as strand discrimination marker we found that the high level of dI removal in polA-D424A was due to strand loss (more than 60%) associated with incomplete repair. Thus, pol I proofreading exonuclease is the major function responsible for dI lesion removal after endoV nicking both in vitro and in vivo. Finally, using MALDI-TOF to analyze single-nucleotide extension product we show that the pol I proofreading exonuclease excises only 2-nt 5′ upstream of endo V incision site further honing the role of pol I in the endoV dI dependent repair pathway.

Copyright © 2018 Elsevier B.V. All rights reserved.

KEYWORDS

DNA polymerase I 3′-5′; DNA repair; Deoxyinosine; Endonuclease V repair; In vivo repair assay; MALDI-TOF mass spectrometry; Reconstitution repair in vitro; Repair mechanism; exonuclease

Title

DNA polymerase I proofreading exonuclease activity is required for endonuclease V repair pathway both in vitro and in vivo.

Author

Su KY1, Lin LI1, Goodman SD2, Yen RS3, Wu CY3, Chang WC3, Yang YC1, Cheng WC4, Fang WH5.

Publish date

2018 Apr

PMID

27194215

Abstract

Aptamers can be chemically modified to enhance nuclease resistance and increase target affinity. In this study, we performed chemical modification of 2′-deoxyinosine in AS1411, an anti-proliferative G-rich oligodeoxynucleotide aptamer, which binds selectively to the nucleolin protein. Its function was augmented when 2′-deoxyinosine was incorporated at positions 12, 13, 15, and 24 of AS1411, respectively. In addition, double incorporation of 2′-deoxyinosine at positions 12 and 24 (FAN-1224dI), 13 and 24 (FAN-1324dI), and 15 and 24 (FAN-1524dI) promoted G-quartet formation, as well as inhibition of DNA replication and tumor cell growth, and induced S-phase cell cycle arrest. In further animal experiments, FAN-1224dI, FAN-1324dI and FAN-1524dI resulted in enhanced treatment effects than AS1411 alone. These results suggested that the position and number of modification substituents in AS1411 are critical parameters to improve the diagnostic and therapeutic function of the aptamer. Structural investigations of the FAN-1524dI/nucleolin complex structure, using molecular dynamics simulation, revealed the critical interactions involving nucleolin and 2′-dI incorporated AS1411 compared with AS1411 alone. These findings augment understanding of the role of 2′-deoxyinosine moieties in interactive binding processes.

Title

Bioactivity of 2'-deoxyinosine-incorporated aptamer AS1411.

Author

Fan X1, Sun L2, Wu Y1, Zhang L1, Yang Z1.

Publish date

2016 May 19