(-)-3-(N-Methylamino)-1-(2-thienyl)-1-propanol/3-Methylamino-1-(2-thienyl)-1-propanol/(αS)-α-[2-(Methylamino)ethyl]-2-thiophenemethanol/(S)-3-(Methylamino)-1-(thiophen-2-yl)propan-1-ol/2-Thiophenemethanol, α-[2-(methylamino)ethyl]-, (αS)-/(1S)-3-(Methylamino)-1-(2-thienyl)propan-1-ol/(S)-(-)-3-(N-Methylamino)-1-(2-thienyl)-1-propanol/(S)-3-(MethylaMino)-1-(2-thienyl)-1-propanol/(S)-2-[3-(Methylamino)-1-hydroxypropyl]thiophene/(1S)-3-(Methylamino)-1-(2-thienyl)-1-propanol/(1S)-3-(methylamino)-1-thiophen-2-ylpropan-1-ol/(S)-3-METHYLAMINO-1-(THIOPHENE-2-YL)-PROPAN-1-OL
294.3±35.0 °C at 760 mmHg
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Half of Metis citizens, compared to less than 10 % of the general population of Ontario, reside in northern regions, with little access to bone mineral density (BMD) testing. Metis citizens had lower sex-specific and age-standardized rates of BMD testing, yet similar rates of fracture (both sexes) and pharmacotherapy (women only).
To examine osteoporosis management and common osteoporosis-related fractures among Metis citizens compared to the general population of older adults residing in Ontario.
We linked healthcare (medical and pharmacy) utilization and administrative (demographic) databases with the Metis Nation of Ontario citizenship registry to estimate osteoporosis management (bone mineral density [BMD] testing, pharmacotherapy) and fractures (hip, humerus, radius/ulna) among adults aged ≥50 years, from April 1, 2006 to March 31, 2011. Pharmacotherapy data were limited to residents aged ≥65 years. Sex-specific and age-standardized rates were compared between the Metis and the general population. Multivariable logistic regression was used to compare rates of BMD testing after controlling for differences in age and region of residence between the Metis and the general population.
We studied 4219 Metis citizens (55 % men), and 140 (3 %) experienced a fracture. Half of Metis citizens, compared to less than 10 % of the general population of Ontario, resided in northern regions. We identified significantly lower sex-specific and age-standardized rates of BMD testing among Metis compared to the general population, yet found little difference in fracture rates (both sexes) or pharmacotherapy (women only). Differences in BMD testing disappeared after adjusting for region of residence among women yet remained significant among men.
Despite finding significantly lower rates of osteoporosis management among men, Metis men and women were found to have similar age-standardized fracture rates to the general population.
Aboriginal, Fracture, Management, Metis, Osteoporosis, Pharmacotherapy
Osteoporosis management and fractures in the Metis of Ontario, Canada
Racquel Jandoc, Nathaniel Jembere, Saba Khan, Storm J. Russell, Yvon Allard, and Suzanne M. Cadarettecorresponding author
The family Stachybotriaceae was recently introduced to include the genera Myrothecium, Peethambara and Stachybotrys. Members of this family include important plant and human pathogens, as well as several species used in industrial and commercial applications as biodegraders and biocontrol agents. However, the generic boundaries in Stachybotriaceae are still poorly defined, as type material and sequence data are not readily available for taxonomic studies. To address this issue, we performed multi-locus phylogenetic analyses using partial gene sequences of the 28S large subunit (LSU), the internal transcribed spacer regions and intervening 5.8S nrRNA (ITS), the RNA polymerase II second largest subunit (rpb2), calmodulin (cmdA), translation elongation factor 1-alpha (tef1) and β-tubulin (tub2) for all available type and authentic strains. Supported by morphological characters these data resolved 33 genera in the Stachybotriaceae. These included the nine already established genera Albosynnema, Alfaria, Didymostilbe, Myrothecium, Parasarcopodium, Peethambara, Septomyrothecium, Stachybotrys and Xepicula. At the same time the generic names Melanopsamma, Memnoniella and Virgatospora were resurrected. Phylogenetic inference further showed that both the genera Myrothecium and Stachybotrys are polyphyletic resulting in the introduction of 13 new genera with myrothecium-like morphology and eight new genera with stachybotrys-like morphology.
biodegraders, generic concept, human and plant pathogens, indoor mycobiota, multi-gene phylogeny, species concept, taxonomy
Generic hyper-diversity in Stachybotriaceae
L. Lombard, 1 J. Houbraken, 1 C. Decock, 2 R.A. Samson, 1 M. Meijer, 1 M. Reblova, 3 J.Z. Groenewald, 1 and P.W. Crous 1 , 4 , 5 , 6
MicroRNAs (miRNAs) are endogenous small RNAs which play important negative regulatory roles at both the transcriptional and post-transcriptional levels in plants. Wheat is the most commonly cultivated plant species worldwide. In this study, RNA-seq analysis was used to examine the expression profiles of miRNA in the spikelets of photo-thermosenisitive genic male sterile (PTGMS) wheat line BS366 during male fertility transition. Through mapping on their corresponding precursors, 917-7,762 novel miRNAs were found in six libraries. Six novel miRNAs were selected for examination of their secondary structures and confirmation by stem-loop RT-PCR. In a differential expression analysis, 20, 22, and 58 known miRNAs exhibited significant differential expression between developmental stages 1 (secondary sporogenous cells had formed), 2 (all cells layers were present and mitosis had ceased), and 3 (meiotic division stage), respectively, of fertile and sterile plants. Some of these differential expressed miRNAs, such as tae-miR156, tae-miR164, tae-miR171, and tae-miR172, were shown to be associated with their targets. These targets were previously reported to be related to pollen development and/or male sterility, indicating that these miRNAs and their targets may be involved in the regulation of male fertility transition in the PTGMS wheat line BS366. Furthermore, target genes of miRNA cleavage sites were validated by degradome sequencing. In this study, a possible signal model for the miRNA-mediated signaling pathway during the process of male fertility transition in the PTGMS wheat line BS366 was developed. This study provides a new perspective for understanding the roles of miRNAs in male fertility in PTGMS lines of wheat.
Triticum aestivum L., photoperiod thermo-sensitive genic male sterility (PTGMS), miRNA, fertile transformation, RNA-seq
Uncovering Male Fertility Transition Responsive miRNA in a Wheat Photo-Thermosensitive Genic Male Sterile Line by Deep Sequencing and Degradome Analysis
Jian-Fang Bai,1,2,† Yu-Kun Wang,1,2,† Peng Wang,1,2,3,† Wen-Jing Duan,1,2,4 Shao-Hua Yuan,1,2 Hui Sun,1,2 Guo-Liang Yuan,1,2 Jing-Xiu Ma,1,2 Na Wang,1,2 Feng-Ting Zhang,1,2 Li-Ping Zhang,1,2,* and Chang-Ping Zhao1,2,*