3-(2-Hydroxy-3,4-dimethoxyphenyl)-3,4-dihydro-2H-chromen-7-yl β-D-glucopyranoside/7,2'-Dihydroxy-3',4'-dimethoxyisoflavan-7-O-Beta-D-glucopyranoside/β-D-Glucopyranoside, 3,4-dihydro-3-(2-hydroxy-3,4-dimethoxyphenyl)-2H-1-benzopyran-7-yl/3,4-Dihydro-3-(2-hydroxy-3,4-dimethoxyphenyl)-2H-1-benzopyran-7-yl beta-D-glucopyranoside
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
641.5±55.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:94367-43-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Buyang Huanwu decoction (BHD) was reported to exert angiogenesis-promoting effects, but its active ingredients remain unknown. In this study, we developed a method to screen potential angiogenesis-promoting compounds in BHD, which involved biospecific isolation using live rat brain microvascular endothelial cells (rBMECs) and characterization using solid-phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Six compounds showed binding affinity to rBMECs and were further identified as 6-hydroxykaempferol-di-O-glucoside, paeoniflorin, calycosin-7-O-β-D-glucoside, galloylpaeoniflorin, formononetin-7-O-β-D-glucoside, and (3R)-7,2′-hydroxy-3′,4′-dimethoxy-isoflavan. The results indicated that five of them except 6-hydroxykaempferol-di-O-glucoside showed a protective effect against oxygen glucose deprivation/reperfusion injury in rBMECs and upregulated the secretion of vascular endothelial growth factor and basic fibroblast growth factor, suggesting a mechanism underlying their angiogenic activity. Our findings suggest that biospecific live cell-based isolation combined with SPE and HPLC-MS/MS is an effective method for screening potential bioactive components in traditional Chinese medicines.
Copyright ? 2017 Elsevier B.V. All rights reserved.
Angiogenesis; Biospecific live cell-based isolation; Buyang Huanwu decoction; Oxygen-glucose deprivation and reperfusion; Solid-phase extraction; Traditional Chinese medicines
Biospecific isolation and characterization of angiogenesis-promoting ingredients in Buyang Huanwu decoction using affinity chromatography on rat brain microvascular endothelial cells combined with solid-phase extraction, and HPLC-MS/MS.
Liao F1, Meng Y2, Zheng H2, He D2, Shen X2, Yu J2, Wu Y2, Wang L3.
2018 Mar 1;
Six compounds were isolated from the root of Astragalus chrysopterus Bunge. One of them was identified as a new triterpenoid glycoside, named astrachrysoside A. Its structure was elucidated as 3-O-[alpha-L-rhamnopyranosyl (1—-2) beta-D-xylopyranosyl]-cycloastragenol on the basis of physicochemical evidence and chemical reactions. The others were shown to be known compounds, astrasieversianin XV (II), 7,2′-dihydroxy-3′,4′-dimethoxy-isoflavane-7-O-beta-D-glucoside (III), soyasaponin I, daucosterol and beta-sitosterol. Full assignment of the chemical shift signals of II and III were established by various 2D-NMR techniques.
[The structure of astrachrysosid A and the study of 2D-NMR on astrasieversianin XV and 7,2'-dihydroxy-3',4'-dimethoxy-isoflavane-7-O-beta-D-glycoside].
Wang HK1, He K, Xu HX, Zhang ZL, Wang YF, Kikuchi T, Tezuka Y.