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5′-Cytidylic acid

$43

  • Brand : BIOFRON

  • Catalogue Number : BF-C3026

  • Specification : 98%

  • CAS number : 63-37-6

  • Formula : C9H14N3O8P

  • Molecular Weight : 323.198

  • Volume : 100mg

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Catalogue Number

BF-C3026

Analysis Method

HPLC,NMR,MS

Specification

98%

Storage

2-8°C

Molecular Weight

323.198

Appearance

powder

Botanical Source

Structure Type

Nucleosiede

Category

SMILES

Synonyms

IUPAC Name

Density

2.2±0.1 g/cm3

Solubility

Flash Point

354.6±34.3 °C

Boiling Point

662.8±65.0 °C at 760 mmHg

Melting Point

~222 °C (dec.)

InChl

InChl Key

WGK Germany

RID/ADR

HS Code Reference

2933590000

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:63-37-6) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

PMID

32567283

Abstract

Uridine-cytidine kinase, an important catalyst in the compensation pathway of nucleotide metabolism, can catalyze the phosphorylation reaction of cytidine to 5′-cytidine monophosphate (CMP), but the reaction needs NTP as the phosphate donor. To increase the production efficiency of CMP, uridine-cytidine kinase gene from Thermus thermophilus HB8 and polyphosphate kinase gene from Rhodobacter sphaeroides were cloned and expressed in Escherichia coli BL21(DE3). Uridine-cytidine kinase was used for the generation of CMP from cytidine and ATP, and polyphosphate kinase was used for the regeneration of ATP. Then, the D403 metal chelate resin was used to adsorb Ni²⁺ to form an immobilized carrier, and the immobilized carrier was specifically combined with the recombinant enzymes to form the immobilized enzymes. Finally, single-factor optimization experiment was carried out to determine the reaction conditions of the immobilized enzyme. At 30 °C and pH 8.0, 60 mmol/L cytidine and 0.5 mmol/L ATP were used as substrates to achieve 5 batches of high-efficiency continuous catalytic reaction, and the average molar yield of CMP reached 91.2%. The above method has the advantages of low reaction cost, high product yield and high enzyme utilization rate, and has good applied value for industrial production.

KEYWORDS

5′-cytidine monophosphate; immobilized enzyme; polyphosphate kinase; uridine-cytidine kinase.

Title

[Concomitant use of immobilized uridine-cytidine kinase and polyphosphate kinase for 5'-cytidine monophosphate production]

Author

Sijia Wu 1, Jie Li 1, Chenlong Hu 1, Junyu Tian 1, Tong Zhang 1, Ning Chen 1, Xiaoguang Fan 1

Publish date

2020 May 25

PMID

31712916

Abstract

Isothermal titration calorimetry (ITC) can benefit from operating in miniaturized devices as they enable quantitative, low-cost measurements with reduced analysis time and reagents consumption. However, most of the existing devices that offer ITC capabilities either do not yet allow proper control of reaction conditions or are limited by issues such as evaporation or surface adsorption caused inaccurate solution concentration information and unintended changes in biomolecular properties because of aggregation. In this paper, we present a microdevice that combines 3D-printed microfluidic structures with a polymer-based MEMS thermoelectric sensor to enable quantitative ITC measurements of biomolecular interactions. Benefitting from the geometric flexibility of 3D-printing, the microfluidic design features calorimetric chambers in a differential cantilever configuration that improves the thermal insulation and reduces the thermal mass of the implementing device. Also, 3D-printing microfluidic structures use non-permeable materials to avoid potential adsorption. Finally, the robustness of the polymeric MEMS sensor chip allows the device to be assembled reversibly and leak-free, and hence reusable. We demonstrate the utility of the device by quantitative ITC characterization of a biomolecular binding system, ribonuclease A (RNase A) bind with cytidine 2′-monophosphate (2’CMP) down to a practically useful sample concentration of 0.2 mM. The thermodynamic parameters of the binding system, including the stoichiometry, equilibrium binding constant, and enthalpy change are obtained and found to agree with values previously reported in the literature.

KEYWORDS

3D-printed microfluidic structures; Isothermal titration calorimeter; MEMS thermoelectric sensor; Polymer substrate.

Title

Isothermal titration calorimetry in a 3D-printed microdevice

Author

Yuan Jia 1 2, Chao Su 2 3, Maogang He 4, Kun Liu 5, Hao Sun 6, Qiao Lin 7

Publish date

2019 Nov 11

PMID

31691999

Abstract

The biosynthesis of sialic acid (Neu5Ac) leads to the intracellular production of cytidine-5′-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac), the active sialic acid donor to nascent glycans (glycoproteins and glycolipids) in the Golgi. UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase myopathy is a rare autosomal recessive muscular disease characterized by progressive muscle weakness and atrophy. To quantify the intracellular levels of CMP-Neu5Ac as well as N-acetylmannosamine (ManNAc) and Neu5Ac in human leukocytes, we developed and validated robust liquid chromatography-tandem mass spectrometry methods. A fit-for-purpose approach was implemented for method validation. Hydrophilic interaction chromatography was used to retain three hydrophilic analytes. The human leukocyte pellets were lysed and extracted in a methanol-water mixture and the leukocyte extract was used for LC-MS/MS analysis. The lower limits of quantitation for ManNAc, Neu5Ac and CMP-Neu5Ac were 25.0, 25.0 and 10.0 ng/ml, respectively. These validated methods were applied to a clinical study.

KEYWORDS

LC-MS/MS; cytidine-5′-monophospho-N-acetylneuraminic acid; human leukocytes; hydrophilic interaction chromatography.

Title

Quantitation of cytidine-5'-monophospho-N-acetylneuraminic acid in human leukocytes using LC-MS/MS: method development and validation

Author

Meng Fang 1, Xin Xu 2, Michael Zhang 1, Yifan Shi 1, Guodong Gu 1, Wanjing Liu 1, Bradley Class 3, Carla Ciccone 3, William A Gahl 3, Marjan Huizing 3, Nuria Carrillo 3, Amy Q Wang 2

Publish date

2020 Feb;


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