Quinolin-6-amine dihydrochloride/6-Quinolinamine dihydrochloride/quinolin-6-amine/6-Aminoquinoline Dihydrochloride/6-Quinolinamine, hydrochloride (1:2)
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provides coniferyl ferulate(CAS#:580-15-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Fatty acids (FAs) have been selectively derivatized with a fluorescent tag, 6-aminoquinoline (6AQ), which yielded fluorescent FA-6AQ derivatives that have excitation (λexc = 270 nm) and emission (λemi = 495 nm) wavelengths that are farther apart. This precolumn derivatization is characterized by its simplicity occurring at room temperature between the carboxylic acid group of the FA and the amino group of 6AQ in the presence of a nonaqueous soluble carbodiimide coupling agent such as the N,N´-dicyclohexylcarbodiimide. The FAs extracts are readily derivatized in chloroform and can be analyzed without any further sample cleanup that minimizes sample loss. The FA-6AQ derivatives derived from standard FAs as well as from extracted FAs from food samples were separated by reversed phase chromatography on a homemade naphthyl methacrylate monolithic (NMM) column and C4 silica-based column. While the NMM column provided excellent separation for saturated FA-6AQ derivatives, the C4 silica column was able to separate simultaneously saturated and unsaturated FA-6AQ derivatives. The MNN column permitted the analysis and quantitation of the saturated FA-6AQ derivatives extracted from coconut oil. The C4 column provided the selectivity needed to analyze and quantify saturated and unsaturated derivatized with 6AQ and extracted from meat. The limits of detection and quantitation were 5 and 20 nM, respectively, with a linear dynamic range extending from 20 nM to 40 μM. The 40 μM upper limit was due to the limited solubility of the FA-6AQ derivatives in the diluting mobile phase, which is the initial mobile phase used in gradient runs.
Butyl-silica column; Fatty acids; Fluorescent tag; HPLC; Naphthyl monolithic column.
Selective Precolumn Derivatization of Fatty Acids With the Fluorescent Tag 6-aminoquinoline and Their Determination in Some Food Samples by Reversed-Phase Chromatography
Murthy Jonnada 1, Guadalupe Davila El Rassi 2, Ziad El Rassi 1
Synthesis of 6-methoxy-4-aminoquinoline
Phospholipase C isozymes (PLCs) catalyze the conversion of the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) into two second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. This family of enzymes are key signaling proteins that regulate the physiological responses of many extracellular stimuli such as hormones, neurotransmitters, and growth factors. Aberrant regulation of PLCs has been implicated in various diseases including cancer and Alzheimer’s disease. How, when, and where PLCs are activated under different cellular contexts are still largely unknown. We have developed a fluorogenic PLC reporter, WH-15, that can be cleaved in a cascade reaction to generate fluorescent 6-aminoquinoline. When applied in enzymatic assays with either pure PLCs or cell lysates, this reporter displays more than a 20-fold fluorescence enhancement in response to PLC activity. Under assay conditions, WH-15 has comparable K(m) and V(max) with the endogenous PIP(2). This novel reporter will likely find broad applications that vary from imaging PLC activity in live cells to high-throughput screening of PLC inhibitors.
A Fluorogenic, Small Molecule Reporter for Mammalian Phospholipase C Isozymes
Weigang Huang 1, Stephanie N Hicks, John Sondek, Qisheng Zhang
2011 Mar 18