Cefadroxil Related Compound B/(6R,7R)-7-Amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid/5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 7-amino-3-methyl-8-oxo-, (6R,7R)-7beta-aminodeacetoxycephalosporanic acid/7-Aminodesacetoxycephalosporanic acid/7-ADCA/Cefalexin Impurity 2
517.6±50.0 °C at 760 mmHg
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provides coniferyl ferulate(CAS#:22252-43-3) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
7-aminodeacetoxycephalosporanic acid (7-ADCA) is a key intermediate of many clinically useful semisynthetic cephalosporins that were traditionally prepared by processes involving chemical ring expansion of penicillin G. Bioconversion of penicillins to cephalosporins using deacetoxycephalosporin C synthase (DAOCS) is an alternative and environmentally friendly process for 7-ADCA production. Arnold Demain and co-workers pioneered such a process. Later, protein engineering efforts to improve the substrate specificity and catalytic efficiency of DAOCS for penicillins have been made by many groups, and a whole cell process using Escherichia coli for bioconversion of penicillins has been developed.
7-aminodeacetoxycephalosporanic acid (7-ADCA); Bioconversion; Deacetoxycephalosporin C synthase (DAOCS); Penicillin G; Penicillin expandase.
Engineering Deacetoxycephalosporin C Synthase as a Catalyst for the Bioconversion of Penicillins
Keqiang Fan 1, Baixue Lin 2, Yong Tao 2, Keqian Yang 3
[Achievements in the Field of Obtaining and Studying New Semisynthetic Cephalosporin Antibiotics. Research in the Field of Directed Cephalosporin Synthesis]
G S Rozenfel'd, S M Navashin
Cephalosporins are widely used antibiotics owing to their broad activity spectra and low toxicity. Many of these medically important compounds are made chemically from 7-aminodeacetoxycephalosporanic acid. At present, this intermediate is made by synthetic ring-expansion of the inexpensive penicillin G to form G-7-ADCA, followed by enzymatic removal of the side chain to obtain 7-ADCA. The chemical synthetic process is expensive, complicated and environmentally unfriendly. Environmentally compatible enzymatic process is favorable compared with chemical synthesis. In our previous research, metabolic engineered Escherichia coli strain (H7/PG15) was constructed and used as whole-cell biocatalyst for the production of G-7-ADC with penicillin G as substrate. The whole-cell biocatalysis was studied by single factor experiment, including the composition of substrates and the conversion conditions (OD600, pH, concentration of penicillin G, MOPS, glucose, time and FeSO4). After optimization, 15 mmol/L of G-7-ADCA was obtained. The process is convenient, efficient and economic. This work would facilitate the industrial manufacturing and further product research.
[Optimization of Whole-Cell Biocatalysis for Phenylacetyl- 7-aminodeacetoxycephalosporanic Acid Production]
Jinheng Fu, Jian Zhao, Baixue Lin, Yang Xu, Yong Tao