1H-Pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione, 4-ethyl-4-hydroxy-10-methoxy-, (4S)-/10-methoxycamptothecin/9-methoxycamtothecin/(4S)-4-Ethyl-4-hydroxy-10-methoxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione/Camptothecin,9-methoxy
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
773.1±60.0 °C at 760 mmHg
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provides coniferyl ferulate(CAS#:39026-92-1) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression in a variety of organisms, including insects, vertebrates, and plants. miRNAs play important roles in cell development and differentiation as well as in the cellular response to stress and infection. To date, there are limited reports of miRNA identification in mosquitoes, insects that act as essential vectors for the transmission of many human pathogens, including flaviviruses. West Nile virus (WNV) and dengue virus, members of the Flaviviridae family, are primarily transmitted by Aedes and Culex mosquitoes. Using high-throughput deep sequencing, we examined the miRNA repertoire in Ae. albopictus cells and Cx. quinquefasciatus mosquitoes.
We identified a total of 65 miRNAs in the Ae. albopictus C7/10 cell line and 77 miRNAs in Cx. quinquefasciatus mosquitoes, the majority of which are conserved in other insects such as Drosophila melanogaster and Anopheles gambiae. The most highly expressed miRNA in both mosquito species was miR-184, a miRNA conserved from insects to vertebrates. Several previously reported Anopheles miRNAs, including miR-1890 and miR-1891, were also found in Culex and Aedes, and appear to be restricted to mosquitoes. We identified seven novel miRNAs, arising from nine different precursors, in C7/10 cells and Cx. quinquefasciatus mosquitoes, two of which have predicted orthologs in An. gambiae. Several of these novel miRNAs reside within a ~350 nt long cluster present in both Aedes and Culex. miRNA expression was confirmed by primer extension analysis. To determine whether flavivirus infection affects miRNA expression, we infected female Culex mosquitoes with WNV. Two miRNAs, miR-92 and miR-989, showed significant changes in expression levels following WNV infection.
Aedes and Culex mosquitoes are important flavivirus vectors. Recent advances in both mosquito genomics and high-throughput sequencing technologies enabled us to interrogate the miRNA profile in these two species. Here, we provide evidence for over 60 conserved and seven novel mosquito miRNAs, expanding upon our current understanding of insect miRNAs. Undoubtedly, some of the miRNAs identified will have roles not only in mosquito development, but also in mediating viral infection in the mosquito host.
Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
Rebecca L Skalsky,1 Dana L Vanlandingham,3 Frank Scholle,2 Stephen Higgs,3 and Bryan R Cullencorresponding author1
2010 Feb 18
Menopause timing has a substantial impact on infertility and risk of disease, including breast cancer, but the underlying mechanisms are poorly understood. We report a dual strategy in ~70,000 women to identify common and low-frequency protein-coding variation associated with age at natural menopause (ANM). We identified 44 regions with common variants, including two harbouring additional rare missense alleles of large effect. We found enrichment of signals in/near genes involved in delayed puberty, highlighting the first molecular links between the onset and end of reproductive lifespan. Pathway analyses revealed a major association with DNA damage-response (DDR) genes, including the first common coding variant in BRCA1 associated with any complex trait. Mendelian randomisation analyses supported a causal effect of later ANM on breast cancer risk (~6% risk increase per-year, P=3×10−14), likely mediated by prolonged sex hormone exposure, rather than DDR mechanisms.
Large-scale genomic analyses link reproductive ageing to hypothalamic signaling, breast cancer susceptibility and BRCA1-mediated DNA repair
Felix R. Day,#1 Katherine S. Ruth,#2 Deborah J. Thompson,#3 Kathryn L. Lunetta,4,5 Natalia Pervjakova,6,7 Daniel I. Chasman,8,9 Lisette Stolk,10,11 Hilary K. Finucane,12,13 Patrick Sulem,14 Brendan Bulik-Sullivan,15,16,17 Tõnu Esko,6,18,19,20 Andrew D. Johnson,5 Cathy E. Elks,1 Nora Franceschini,21 Chunyan He,22,23 Elisabeth Altmaier,24,25,26 Jennifer A. Brody,27 Lude L. Franke,28 Jennifer E. Huffman,5,29 Margaux F. Keller,30 Patrick F. McArdle,31 Teresa Nutile,32 Eleonora Porcu,33,34,35 Antonietta Robino,36 Lynda M. Rose,8 Ursula M. Schick,37 Jennifer A. Smith,38 Alexander Teumer,39 Michela Traglia,40 Dragana Vuckovic,36,41 Jie Yao,42 Wei Zhao,38 Eva Albrecht,25 Najaf Amin,43 Tanguy Corre,44,45 Jouke-Jan Hottenga,46 Massimo Mangino,47,48 Albert V. Smith,49,50 Toshiko Tanaka,51 Goncalo Abecasis,35 Irene L. Andrulis,52,53 Hoda Anton-Culver,54 Antonis C. Antoniou,3 Volker Arndt,55 Alice M. Arnold,56 Caterina Barbieri,36,40 Matthias W. Beckmann,57 Alicia Beeghly-Fadiel,58 Javier Benitez,59,60 Leslie Bernstein,61 Suzette J. Bielinski,62 Carl Blomqvist,63 Eric Boerwinkle,64,65 Natalia V. Bogdanova,66 Stig E. Bojesen,67,68 Manjeet K. Bolla,3 Anne-Lise Borresen-Dale,69,70 Thibaud S Boutin,29 Hiltrud Brauch,71,72,73 Hermann Brenner,55,73,74 Thomas Bruning,75 Barbara Burwinkel,76,77 Archie Campbell,78 Harry Campbell,79 Stephen J. Chanock,80 J. Ross Chapman,81 Yii-Der Ida Chen,42 Georgia Chenevix-Trench,82 Fergus J. Couch,83 Andrea D. Coviello,84 Angela Cox,85 Kamila Czene,86 Hatef Darabi,86 Immaculata De Vivo,12,87 Ellen W. Demerath,88 Joe Dennis,3 Peter Devilee,89,90 Thilo Dork,91 Isabel dos-Santos-Silva,92 Alison M. Dunning,93 John D. Eicher,5 Peter A. Fasching,57,94 Jessica D. Faul,95 Jonine Figueroa,96 Dieter Flesch-Janys,97,98 Ilaria Gandin,36,41 Melissa E. Garcia,99 Montserrat Garcia-Closas,100,101 Graham G. Giles,102,103 Giorgia G. Girotto,41 Mark S. Goldberg,104,105 Anna Gonzalez-Neira,59 Mark O. Goodarzi,106 Megan L. Grove,64 Daniel F. Gudbjartsson,14,107 Pascal Guenel,108,109 Xiuqing Guo,42 Christopher A. Haiman,110 Per Hall,86 Ute Hamann,111 Brian E. Henderson,110 Lynne J. Hocking,112 Albert Hofman,43 Georg Homuth,113 Maartje J. Hooning,114 John L. Hopper,102 Frank B. Hu,12,87,115 Jinyan Huang,116 Keith Humphreys,86 David J. Hunter,12,20,87,115 Anna Jakubowska,117 Samuel E. Jones,2 Maria Kabisch,111 David Karasik,9,118 Julia A. Knight,119,120 Ivana Kolcic,121 Charles Kooperberg,37 Veli-Matti Kosma,122,123,124 Jennifer Kriebel,24,26,125 Vessela Kristensen,69,70,126 Diether Lambrechts,127,128 Claudia Langenberg,1 Jingmei Li,86 Xin Li,12 Sara Lindstrom,12 Yongmei Liu,129 Jian’an Luan,1 Jan Lubinski,117 Reedik Magi,6 Arto Mannermaa,122,123,124 Judith Manz,24,26 Sara Margolin,130 Jonathan Marten,29 Nicholas G. Martin,131 Corrado Masciullo,40 Alfons Meindl,132 Kyriaki Michailidou,3 Evelin Mihailov,6 Lili Milani,6 Roger L. Milne,102,103 Martina Muller-Nurasyid,25,133,134 Michael Nalls,135 Ben M. Neale,15,16,17 Heli Nevanlinna,136 Patrick Neven,137 Anne B. Newman,138,139,140 Børge G. Nordestgaard,67,68 Janet E. Olson,62 Sandosh Padmanabhan,141 Paolo Peterlongo,142 Ulrike Peters,37 Astrid Petersmann,143 Julian Peto,92 Paul D.P. Pharoah,3,93 Nicola N. Pirastu,36,41 Ailith Pirie,3 Giorgio Pistis,33,34,35 Ozren Polasek,121 David Porteous,78 Bruce M. Psaty,27,144,145,146 Katri Pylkas,147,148 Paolo Radice,149 Leslie J. Raffel,150,151 Fernando Rivadeneira,10,11,43 Igor Rudan,79 Anja Rudolph,152 Daniela Ruggiero,32 Cinzia F. Sala,40 Serena Sanna,33 Elinor J. Sawyer,153 David Schlessinger,154 Marjanka K. Schmidt,155 Frank Schmidt,113 Rita K. Schmutzler,156,157,158 Minouk J. Schoemaker,100 Robert A. Scott,1 Caroline M. Seynaeve,114 Jacques Simard,159 Rossella Sorice,32 Melissa C. Southey,160 Doris Stockl,26 Konstantin Strauch,25,161 Anthony Swerdlow,100,162 Kent D. Taylor,42 Unnur Thorsteinsdottir,14,50 Amanda E. Toland,163 Ian Tomlinson,81,164 Therese Truong,108,109 Laufey Tryggvadottir,165 Stephen T. Turner,166 Diego Vozzi,36 Qin Wang,3 Melissa Wellons,167 Gonneke Willemsen,46 James F. Wilson,29,79 Robert Winqvist,147,148 Bruce B.H.R. Wolffenbuttel,168,169 Alan F. Wright,29 Drakoulis Yannoukakos,170 Tatijana Zemunik,121 Wei Zheng,58 Marek Zygmunt,171 Sven Bergmann,44,45 Dorret I. Boomsma,46 Julie E. Buring,8,9 Luigi Ferrucci,51 Grant W. Montgomery,131 Vilmundur Gudnason,49,50 Tim D. Spector,47 Cornelia M van Duijn,43 Behrooz Z. Alizadeh,172 Marina Ciullo,32 Laura Crisponi,33 Douglas F. Easton,3,93 Paolo P. Gasparini,36,41 Christian Gieger,24,25,26 Tamara B. Harris,99 Caroline Hayward,29 Sharon L.R. Kardia,38 Peter Kraft,12,173 Barbara McKnight,56 Andres Metspalu,6 Alanna C. Morrison,64 Alex P. Reiner,37,144 Paul M. Ridker,8,9 Jerome I. Rotter,42 Daniela Toniolo,40 Andre G. Uitterlinden,10,11,43 Sheila Ulivi,36 Henry Volzke,39 Nicholas J. Wareham,1 David R. Weir,95 Laura M. Yerges-Armstrong,31 PRACTICAL consortium, kConFab Investigators,174 AOCS Investigators,174 Generation Scotland,175 EPIC-InterAct Consortium, LifeLines Cohort Study, Alkes L. Price,12 Kari Stefansson,14,50 Jenny A. Visser,10 Ken K. Ong,1,176 Jenny Chang-Claude,152 Joanne M. Murabito,5,177,§ John R.B. Perry,1,§# and Anna Murray2,§
2016 May 1.
Background: Dietary flavonoids have beneficial effects on blood pressure in intervention settings, but there is limited information on habitual intake and risk of hypertension in population-based studies.
Objective: We examined the association between habitual flavonoid intake and incident hypertension in a prospective study in men and women.
Design: A total of 87,242 women from the Nurses’ Health Study (NHS) II, 46,672 women from the NHS I, and 23,043 men from the Health Professionals Follow-Up Study (HPFS) participated in the study. Total flavonoid and subclass intakes were calculated from semiquantitative food-frequency questionnaires collected every 4 y by using an updated and extended US Department of Agriculture database.
Results: During 14 y of follow-up, 29,018 cases of hypertension in women and 5629 cases of hypertension in men were reported. In pooled multivariate-adjusted analyses, participants in the highest quintile of anthocyanin intake (predominantly from blueberries and strawberries) had an 8% reduction in risk of hypertension [relative risk (RR): 0.92; 95% CI: 0.86, 0.98; P < 0.03] compared with that for participants in the lowest quintile of anthocyanin intake; the risk reduction was 12% (RR: 0.88; 95% CI: 0.84, 0.93; P < 0.001) in participants ≤60 y of age and 0.96 (0.91, 1.02) in participants >60 y of age (P for age interaction = 0.02). Although intakes of other subclasses were not associated with hypertension, pooled analyses for individual compounds suggested a 5% (95% CI: 0.91, 0.99; P = 0.005) reduction in risk for the highest compared with the lowest quintiles of intake of the flavone apigenin. In participants ≤60 y of age, a 6% (95% CI: 0.88, 0.97; P = 0.002) reduction in risk was observed for the flavan-3-ol catechin when the highest and the lowest quintiles were compared.
Conclusions: Anthocyanins and some flavone and flavan-3-ol compounds may contribute to the prevention of hypertension. These vasodilatory properties may result from specific structural similarities (including the B-ring hydroxylation and methyoxylation pattern).
Habitual intake of flavonoid subclasses and incident hypertension in adults1,2,3
Aedin Cassidy, eilis J O'Reilly, Colin Kay, Laura Sampson, Mary Franz, JP Forman, Gary Curhan, and Eric B Rimm
9-Methoxycamptothecin (MCPT), isolated from Nothapodytes foetida, has antitumor activities through topoisomerase inhibition. 9-Methoxycamptothecin (MCPT) induces strong G2/M arrest and apoptosis in cancer.