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beta-Anhydrouzarigenin

$830

  • Brand : BIOFRON

  • Catalogue Number : AV-B02054

  • Specification : 97%

  • CAS number : 3080-20-4

  • Formula : C23H32O3

  • Molecular Weight : 356.5

  • PUBCHEM ID : 91895411

  • Volume : 5mg

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Catalogue Number

AV-B02054

Analysis Method

HPLC,NMR,MS

Specification

97%

Storage

2-8°C

Molecular Weight

356.5

Appearance

Powder

Botanical Source

Structure Type

Steroids

Category

Standards;Natural Pytochemical;API

SMILES

CC12CCC(CC1CCC3C2CCC4(C3=CCC4C5=CCOC5=O)C)O

Synonyms

Carda-14,20(22)-dienolide, 3-hydroxy-, (3β,5α)-/3-[(3β,5α,17β)-3-Hydroxyandrost-14-en-17-yl]-2(5H)-furanone/(3β,5α)-3-Hydroxycarda-14,20(22)-dienolide/2(5H)-Furanone, 3-[(3β,5α,17β)-3-hydroxyandrost-14-en-17-yl]-/b-Anhydro-epi-digitoxigenin/Beta-Anhydrouzarigenin

IUPAC Name

4-[(3S,5S,8R,9S,10S,13R,17S)-3-hydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]-2H-furan-5-one

Applications

Density

1.2±0.1 g/cm3

Solubility

Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.

Flash Point

217.0±22.9 °C

Boiling Point

537.4±50.0 °C at 760 mmHg

Melting Point

InChl

InChl Key

DPDKGFZJWHLPLO-UENNAJKTSA-N

WGK Germany

RID/ADR

HS Code Reference

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:3080-20-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

No Technical Documents Available For This Product.

PMID

27409075

Title

Proceedings of the 3rd IPLeiria’s International Health Congress Leiria, Portugal. 6-7 May 2016

Author

Catarina Cardoso Tomas,corresponding author Emanuel Oliveira,corresponding author D. Sousa, M. Uba-Chupel, G. Furtado, C. Rocha, A. Teixeira, P. Ferreira, Celeste Alves,corresponding author Stefan Gisin,corresponding author Elisabete Catarino,corresponding author Nelma Carvalho, Tiago Coucelo, Luis Bonfim, Carina Silva, Debora Franco,corresponding author Jes?s Alcoba Gonzalez,corresponding author Helena G. Jardim,corresponding author Rita Silva, Cristina L. Baixinho,corresponding author Mª Helena Presado, Mª Fatima Marques, Mario E. Cardoso, Marina Cunha,corresponding author Joana Mendes, Ana Xavier, Ana Galhardo, Margarida Couto, Jo?o G. Frade,corresponding author Carla Nunes, Jo?o R. Mesquita, Maria S. Nascimento, Guilherme Goncalves, Conceic?o Castro, Alice Martires, Mª Jo?o Monteiro, Conceic?o Rainho,corresponding author Francisco P. Caballero,corresponding author Fatima M. Monago, Jose T. Guerrero, Rocio M. Monago, Africa P. Trigo, Milagros L. Gutierrez, Gemma M. Milanes, Mercedes G. Reina, Ana G. Villanueva, Ana S. PiNero, Isabel R. Aliseda, Francisco B. Ramirez, Andrea Ribeiro,corresponding author Ana Quelhas, Conceic?o Manso, Francisco P. Caballero,corresponding author Jose T. Guerrero, Fatima M. Monago, Rafael B. Santos, Nuria R. Jimenez, Cristina G. NuNez, Inmaculada R. Gomez, Mª Jose L. Fernandez, Laura A. Marquez, Ana L. Moreno, Mª Jesus Tena Huertas, Francisco B. Ramirez, Daniel Seabra,corresponding author Mª Ceu Salvador, Luciene Braga,corresponding author Pedro Parreira, Anabela Salgueiro-Oliveira, Cristina Arreguy-Sena, Bibiana F. Oliveira, Mª Adriana Henriques, Joana Santos,corresponding author Sara Lebre, Alda Marques, Clarinda Festas,corresponding author Sandra Rodrigues, Andrea Ribeiro, Jose Lumini, Ana G. Figueiredo,corresponding author Francisco J. Hernandez-Martinez,corresponding author Liliana Campi, Mª Pino Quintana-Montesdeoca, Juan F. Jimenez-Diaz, Bienvenida C. Rodriguez-De-Vera, Alexandra Parente, Mª Augusta Mata, Ana Mª Pereira, Adilia Fernandes, Manuel Bras,corresponding author Mª Rosario Pinto,corresponding author Pedro Parreira, Marta L. Basto, Ana C. Rei, Lisete M. Monico, Gilberta Sousa,corresponding author Clementina Morna, Otilia Freitas, Gregorio Freitas, Ana Jardim, Rita Vasconcelos, Lina G. Horta, Roger S. Rosa,corresponding author Luis F. Kranz, Rita C. Nugem, Mariana S. Siqueira, Ronaldo Bordin, Rosiane Kniess,corresponding author Josimari T. Lacerda, Joana Guedes,corresponding author Idalina Machado, Sidalina Almeida, Adriano Zilh?o, Helder Alves, oscar Ribeiro, Ana P. Amaral,corresponding author Ana Santos, Joana Monteiro, Mª Clara Rocha, Rui Cruz, Ana P. Amaral,corresponding author Marina Lourenco, Mª Clara Rocha, Rui Cruz, Sandra Antunes, Veronica Mendonca, Isabel Andrade, Nadia Osorio, Ana Valado, Armando Caseiro, Antonio Gabriel, Anabela C. Martins, Fernando Mendes,corresponding author Lidia Cabral,corresponding author Manuela Ferreira, Amadeu Goncalves, Tatiana D. Luz,corresponding author Leonardo Luz, Raul Martins, Alice Morgado, Maria L. Vale-Dias, Rui Porta-Nova,corresponding author Tania C. Fleig,corresponding author eboni M. Reuter, Miriam B. Froemming, Sabrina L. Guerreiro, Lisiane L. Carvalho, Daniel Guedelha,corresponding author P. Coelho, A. Pereira, Antonio Calha,corresponding author Raul Cordeiro, Ana Goncalves,corresponding author Ana Certo, Ana Galv?o, Mª Augusta Mata, Aline Welter,corresponding author Elayne Pereira, Sandra Ribeiro, Marcia Kretzer, Juan-Fernando Jimenez-Diaz,corresponding author Carla Jimenez-Rodriguez, Francisco-Jose Hernandez-Martinez, Bienvenida-Del-Carmen Rodriguez-De-Vera, Alexandre Marques-Rodrigues, Patricia Coelho,corresponding author Tiago Bernardes, Alexandre Pereira, Patricia Sousa,corresponding author Jo?o G. Filho, Nazare Nazario, Marcia Kretzer, Odete Amaral,corresponding author Antonio Garrido, Nelio Veiga, Carla Nunes, Ana R. Pedro, Carlos Pereira, Antonio Almeia, Helder M. Fernandes, Carlos Vasconcelos, Nelson Sousa, Victor M. Reis, M. Jo?o Monteiro, Romeu Mendes,corresponding author Isabel C. Pinto,corresponding author Tania Pires, Jo?o Gama, Vera Preto,corresponding author Norberto Silva, Carlos Magalh?es, Matilde Martins, Mafalda Duarte,corresponding author Constanca Pa?l, Ignacio Martin, Arminda A. Pinheiro,corresponding author Sandra Xavier,corresponding author Julieta Azevedo, Elisabete Bento, Cristiana Marques, Mariana Marques, Antonio Macedo, Ana T. Pereira, Jose P. Almeida, Antonio Almeida, Josiane Alves, Nelson Sousa, Francisco Saavedra, Romeu Mendes,corresponding author Ana S. Maia,corresponding author Michelle T. Oliveira, Anderson R. Sousa, Paulo P. Ferreira, Luci S. Lopes, Eujcely C. Santiago, Silvia Monteiro,corresponding author angelo Jesus,corresponding author Armanda Colaco, Antonio Carvalho, Rita P. Silva, Agostinho Cruz, Ana Ferreira,corresponding author Catarina Marques, Jo?o P. Figueiredo, Susana Paix?o, Ana Ferreira,corresponding author Carla Lopes, Fernando Moreira, Jo?o P. Figueiredo, Ana Ferreira, Diana Ribeiro, Fernando Moreira, Jo?o P. Figueiredo,corresponding author Susana Paix?o, Telma Fernandes, Diogo Amado, Jessica Leal, Marcelo Azevedo, Sonia Ramalho,corresponding author Catarina Mangas, Jaime Ribeiro,corresponding author Rita Goncalves, Amelia F Nunes,corresponding author Ana R. Tuna, Carlos R. Martins, Henriqueta D. Forte, Claudia Costa,corresponding author Jose A. Tenedorio, Paula Santana, J. A. Andrade, J. L. Pinto, C. Campofiorito, S. Nunes, A. Carmo, A. Kaliniczenco, B. Alves, F. Mendes,corresponding author C. Jesus, F. Fonseca, F. Gehrke, Carlos Albuquerque,corresponding author Rita Batista, Madalena Cunha, Antonio Madureira, Oliverio Ribeiro, Rosa Martins, Teresa Madeira,corresponding author Catarina Peixoto-Placido, Nuno Santos, Osvaldo Santos, Astrid Bergland, Asta Bye, Carla Lopes, Violeta Alarc?o, Beatriz Goul?o, Nuno Mendonca, Paulo Nicola, Jo?o G. Clara, Jo?o Gomes,corresponding author Ana Querido, Catarina Tomas, Daniel Carvalho, Marina Cordeiro, Marlene C. Rosa,corresponding author Alda Marques, Daniela Brand?o,corresponding author oscar Ribeiro, Lia Ara?jo, Constanca Pa?l, Beatriz Minghelli,corresponding author Sylvina Richaud, Ana L. Mendes,corresponding author Joana Marta-Sim?es, In?s A. Trindade, Claudia Ferreira, Teresa Carvalho,corresponding author Marina Cunha, Jose Pinto-Gouveia, Morgana C. Fernandes, Roger S. Rosa,corresponding author Rita C. Nugem, Luis F. Kranz, Mariana S. Siqueira, Ronaldo Bordin, Anabela C. Martins,corresponding author Anabela Medeiros, Rafaela Pimentel, Andreia Fernandes, Carlos Mendonca, Isabel Andrade, Susana Andrade, Ruth L. Menezes, Rafael Bravo,corresponding author Marta Miranda, Lierni Ugartemendia, Jose Mª Tena, Francisco L. Perez-Caballero, Lorena Fuentes-Broto, Ana B. Rodriguez, Barriga Carmen, M. A. Carneiro, J. N. Domingues, S. Paix?o, J. Figueiredo, V. B. Nascimento, C. Jesus,corresponding author F Mendes, F. Gehrke, B. Alves, L. Azzalis, F. Fonseca, Ana R. Martins,corresponding author Amelia Nunes, Arminda Jorge, Nelio Veiga,corresponding author Ana Amorim, Andre Silva, Liliana Martinho, Luis Monteiro, Rafael Silva, Carina Coelho, Odete Amaral, In?s Coelho, Carlos Pereira, Andre Correia, Diana Rodrigues, Nidia Marante, Pedro Silva, Sara Carvalho, Andre Rts Araujo, Maximiano Ribeiro, Paula Coutinho, Sandra Ventura, Fatima Roque,corresponding author Cristina Calvo,corresponding author Manoela Reses, Jorge Conde,corresponding author Ana Ferreira, Jo?o Figueiredo, David Silva, Luis Seica, Raquel Soares, Ricardo Mour?o, Teresa Kraus,corresponding author Ana C. Abreu, Jose M. Padilha, J?lia M. Alves, Paulino Sousa,corresponding author Manuel Oliveira, Joana Sousa, Sonia Novais,corresponding author Felismina Mendes, Joana Pinto, Joana Cruz, Alda Marques,corresponding author Hugo Duarte,corresponding author Maria Dos Anjos Dixe, Pedro Sousa, In?s Cruz,corresponding author Fernanda Bastos, Filipe Pereira, Francisco L. Carvalho,corresponding author Teresa T. Oliveira, Vitor R. Raposo, Conceic?o Rainho,corresponding author Jose C. Ribeiro, Isabel Barroso, Vitor Rodrigues, Carmo Neves,corresponding author Teresa C. Oliveira, Barbara Oliveira, Mª Carminda Morais, Pilar Baylina, Rogerio Rodrigues,corresponding author Zaida Azeredo, Coralia Vicente, Helia Dias,corresponding author Margarida Sim-Sim, Pedro Parreira,corresponding author Anabela Salgueiro-Oliveira, Amelia Castilho, Rosa Melo, Jo?o Graveto, Jose Gomes, Marina Vaquinhas, Carla Carvalho, Lisete Monico, Nuno Brito, Cassilda Sarroeira,corresponding author Jose Amendoeira, Fatima Cunha, Anabela Candido, Patricia Fernandes, Helena R. Silva, Elsa Silva, Isabel Barroso,corresponding author Leila Lapa, Cristina Antunes, Ana Goncalves,corresponding author Ana Galv?o, Mª Jose Gomes, Susana R. Escanciano, Maria Freitas,corresponding author Pedro Parreira, Jo?o Mar?co, Ana R. Fernandes, Cremilde Cabral, Samuel Alves, Pedro Sousa,corresponding author Antonio Ferreira,corresponding author Fernanda Principe, Ulla-Maija Sepp?nen, Margarida Ferreira, Maribel Carvalhais, Marilene Silva, Manuela Ferreira,corresponding author Joana Silva, Jessica Neves, Diana Costa, Bruno Santos, Soraia Duarte, Silvia Marques,corresponding author Sonia Ramalho, Isabel Mendes, Clarisse Louro, Eva Menino,corresponding author Maria Dixe, Sara S. Dias, Marina Cordeiro,corresponding author Catarina Tomas, Ana Querido, Daniel Carvalho, Jo?o Gomes, Frederico C. Valim,corresponding author Joyce O. Costa, L?cia G. Bernardes, Helena Prebianchi,corresponding author Marlene Cristina Rosa,corresponding author Narcisa Goncalves,corresponding author Maria M. Martins, Paulina Kurcgant, Andre Vieira,corresponding author Sandrina Bento, Sergio Deodato, Isabel Rabiais,corresponding author Laura Reis,corresponding author Ana Torres, Sergio Soares,corresponding author Margarida Ferreira, Pedro Graca, Ceu Leit?o, Renato Abreu, Fernando Bellem, Ana Almeida, Edna Ribeiro-Varandas, Ana Tavares, Jo?o G. Frade,corresponding author Carolina Henriques, Eva Menino, Clarisse Louro, Celia Jord?o, Sofia Neco,corresponding author Carminda Morais, Pedro Ferreira, Carla R. Silva,corresponding author Alice Brito, Antonia Silva, Hugo Duarte,corresponding author Maria Dos Anjos Dixe, Pedro Sousa, Gabriela Postolache,corresponding author Raul Oliveira, Isabel Moreira, Luisa Pedro, Sonia Vicente, Samuel Domingos, Octavian Postolache, Darlen Silva,corresponding author Jo?o G. Filho, Nazare Nazario, Marcia Kretzer, Dulcineia Schneider, Fatima M. Marques,corresponding author Pedro Parreira,corresponding author Carla Carvalho, Lisete M. Monico, Carlos Pinto, Sara Vicente, S?o Jo?o Breda, Jose H. Gomes,corresponding author Rosa Melo, Pedro Parreira, Anabela Salgueiro, Jo?o Graveto, Marina Vaquinhas, Amelia Castilho, angelo Jesus,corresponding author Nuno Duarte, Jose C. Lopes, Helder Nunes, Agostinho Cruz, Anabela Salgueiro-Oliveira,corresponding author Pedro Parreira, Marta L. Basto, Luciene M. Braga, Antonio Ferreira,corresponding author Beatriz Ara?jo, Jose M. Alves, Margarida Ferreira, Maribel Carvalhais, Marilene Silva, Sonia Novais, Ana S. Sousa,corresponding author Candida Ferrito, Pedro L. Ferreira, Alexandre Rodrigues, Margarida Ferreira, Isabel Oliveira,corresponding author Manuela Ferreira,corresponding author Jessica Neves, Diana Costa, Soraia Duarte, Joana Silva, Bruno Santos, Cristina Martins,corresponding author Ana P. Macedo, Odete Ara?jo, Claudia Augusto, Fatima Braga, Lisa Gomes, Maria A. Silva, Rafaela Rosario, Luis Pimenta, Diana Carreira, Patricia Teles, Teresa Barros,corresponding author Catarina Tomas,corresponding author Ana Querido, Daniel Carvalho, Jo?o Gomes, Marina Cordeiro, Daniel Carvalho,corresponding author Ana Querido, Catarina Tomas, Jo?o Gomes, Marina Cordeiro, Cristina Jacome,corresponding author Alda Marques, Sylvie Capelas, Andreia Hall, Dina Alves, Marisa Lousada,corresponding author Mª Helena Loureiro,corresponding author Ana Camarneiro, Margarida Silva, Aida Mendes, Ana Pedreiro, Anne G.Silva,corresponding author Elza S. Coelho, Flavio Melo,corresponding author Fernando Ribeiro, Rui Torres, Rui Costa, Tania Pinho, Cristina Jacome, Alda Marques,corresponding author Barbara Cruz, Daniel Seabra,corresponding author Diogo Carreiras, Maria Ventura, x Cruz,corresponding author Dina Brooks, Alda Marques, M Rosario Pinto,corresponding author Pedro Parreira, Marta Lima-Basto, Miguel Neves, Lisete M. Monico, Carla Bizarro,corresponding author Marina Cunha, Ana Galhardo, Couto Margarida, Ana P. Amorim, Eduardo Silva, Susana Cruz,corresponding author Jose M. Padilha, Jorge Valente, Jose T. Guerrero,corresponding author Francisco P. Caballero, Rafael B. Santos, Estefania P. Gonzalez, Fatima M. Monago, Lierni U. Ugalde, Marta M. Velez, Maria J. Tena, Jose T. Guerrero, Rafael Bravo,corresponding author Francisco L. Perez-Caballero, Isabel A. Becerra, Mª Elizabeth Agudelo, Guadalupe Acedo, Roberto Bajo, Isabel Malheiro,corresponding author Filomena Gaspar, Luisa Barros, Guilherme Furtado,corresponding author Mateus Uba-Chupel, Mariana Marques, Luis Rama, Margarida Braga, Jose P. Ferreira, Ana Mª Teixeira, Jo?o Cruz,corresponding author Tiago Barbosa, angela Sim?es, Luis Coelho, Alexandre Rodrigues,corresponding author Juan-Fernando Jimenez-Diaz, Francisco Martinez-Hernandez, Bienvenida Rodriguez-De-Vera, Pedro Ferreira, Alexandrina Rodrigues, Andre Ramalho,corresponding author Jo?o Petrica, Pedro Mendes, Jo?o Serrano, In?s Santo, Antonio Rosado, Paula Mendonca,corresponding author Katia Freitas, Dora Ferreira,corresponding author Antonio Brito, Renato Fernandes, Sofia Gomes,corresponding author Fernando Moreira, Claudia Pinho, Rita Oliveira, Ana I. Oliveira, Paula Mendonca,corresponding author Ana P. Casimiro, Patricia Martins, Iryna Silva, Diana Evangelista, Catarina Leit?o,corresponding author Fabia Velosa, Nelio Carecho, Luis Coelho, Eva Menino,corresponding author Anjos Dixe, Helena Catarino, Fatima Soares, Ester Gama, Clementina Gordo, Eliana Moreira, Cristiana Mid?es, Marlene Santos,corresponding author Sara Machado, Vania P. Oliveira, Marlene Santos,corresponding author Ana Querido,corresponding author Anjos Dixe, Rita Marques, Zaida Charepe, Ana Antunes, Sofia Santos,corresponding author Marlene C. Rosa,corresponding author Marlene C. Rosa,corresponding author Silvana F. Marques, Beatriz Minghelli,corresponding author Eulalia CaroMinghelli, Mª Jose Luis,corresponding author Teresa Brand?o, Pedro Mendes,corresponding author Daniel Marinho, Jo?o Petrica, Diogo Monteiro, Rui Paulo, Jo?o Serrano, In?s Santo, Lina Monteiro, Fatima Ramalho, Rita Santos-Rocha, Sonia Morgado, Teresa Bento,corresponding author Gilberta Sousa,corresponding author Otilia Freitas, Isabel Silva, Gregorio Freitas, Clementina Morna, Rita Vasconcelos, Tatiana Azevedo,corresponding author Salete Soares, Jacinta Pisco, Paulo P. Ferreira,corresponding author Efrain O. Olszewer, Michelle T. Oliveira, Anderson R. Sousa, Ana S. Maia, Sebasti?o T. Oliveira, Erica Santos, Ana I. Oliveira, Carla Maia, Fernando Moreira, Joana Santos, Maria F. Mendes, Rita F. Oliveira, Claudia Pinho,corresponding author Eduarda Barreira,corresponding author Ana Pereira, Josiana A. Vaz, Andre Novo, Luis D. Silva,corresponding author Bruno Maia, Eduardo Ferreira, Filipa Pires, Renato Andrade, Luis Camarinha, Luis D. Silva,corresponding author Bruno Maia, Eduardo Ferreira, Filipa Pires, Renato Andrade, Luis Camarinha, Ana F. Cesar,corresponding author Mariana Poco, David Ventura, Raquel Loura, Pedro Gomes, Catarina Gomes, Claudia Silva, Elsa Melo, Jo?o Lindo, Joana Domingos, Zaida Mendes, Susana Poeta, Tiago Carvalho, Catarina Tomas, Helena Catarino, Mª Anjos Dixe,corresponding author Andre Ramalho,corresponding author Antonio Rosado, Pedro Mendes, Rui Paulo, In?s Garcia, Jo?o Petrica, Sandra Rodrigues,corresponding author Rui Meneses, Carlos Afonso, Luis Faria, Aderito Seixas, Marina Cordeiro,corresponding author Paulo Granjo, Jose C. Gomes, Nelba R. Souza,corresponding author Guilherme E. Furtado, Saulo V. Rocha, Paula Silva, Joana Carvalho, Marina Ana Morais,corresponding author Sofia Santos, Paula Lebre, Ana Antunes, Antonio Calha,corresponding author Ana Xavier,corresponding author Marina Cunha, Jose Pinto-Gouveia, Liana Alencar, Madalena Cunha,corresponding author Antonio Madureira, Ilda Cardoso,corresponding author Ana Galhardo, Fernanda Daniel, Vitor Rodrigues, Leonardo Luz,corresponding author Tatiana Luz, Mauricio R. Ramos, Dayse C. Medeiros, Bruno M. Carmo, Andre Seabra, Cristina Padez, Manuel C. Silva, Antonio Rodrigues,corresponding author Patricia Coelho, Alexandre Coelho, Madson Caminha,corresponding author Filipe Matheus, Elenice Mendes, Jony Correia, Marcia Kretzer, Francisco J. Hernandez-Martinez,corresponding author Juan F. Jimenez-Diaz, Bienvendida C. Rodriguez-De-Vera, Carla Jimenez-Rodriguez, Yadira Armas-Gonzalez, Catia Rodrigues, catialexrodrig@gmail.com,corresponding author Rosa Pedroso, Jennifer Apolinario-Hagen,corresponding author Viktor Vehreschild, Milene Veloso,corresponding author Celina Magalh?es, Isabel Cabral, Maira Ferraz, Filipe Nave,corresponding author Emilia Costa, Filomena Matos, Jose Pacheco, Antonio Dias,corresponding author Carlos Pereira, Jo?o Duarte, Madalena Cunha, Daniel Silva, Lisete M. Monico,corresponding author Valentim R. Alferes, Mª S?o Jo?o Br?da, Carla Carvalho, Pedro M. Parreira, Mª Carminda Morais,corresponding author Pedro Ferreira, Rui Pimenta, Jose Boavida, Isabel C. Pinto,corresponding author Tania Pires, Catarina Silva, Maria Ribeiro,corresponding author Maria Viega-Branco, Filomena Pereira, Ana Mª Pereira, Fabricia M. Almeida, Gustavo L. Estevez, Sandra Ribeiro, Marcia R. Kretzer,corresponding author Paulo V. Jo?o,corresponding author Paulo Nogueira, Sandra Novais, Ana Pereira, Lara Carneiro, Maria Mota, Rui Cruz,corresponding author Luiz Santiago, Carlos Fontes-Ribeiro, Guilherme Furtado,corresponding author Saulo V. Rocha, Andre P. Coutinho, Jo?o S. Neto, Lelia R. Vasconcelos, Nelba R. Souza, Estelio Dantas, Alexandra Dinis,corresponding author Sergio Carvalho, Paula Castilho, Jose Pinto-Gouveia, Alexandra Sarreira-Santos,corresponding author Amelia Figueiredo, Lurdes Medeiros-Garcia, Paulo Seabra, Rosa Rodrigues,corresponding author Mª Carminda Morais, Paula O. Fernandes, Conceic?o Santiago,corresponding author Mª Henriqueta Figueiredo, Marta L. Basto, Teresa Guimar?es,corresponding author Andre Coelho, Anabela Graca, Ana M. Silva, Ana R. Fonseca, Luz Vale-Dias,corresponding author Barbara Minas, Graciete Franco-Borges, Cristina Sim?es,corresponding author Sofia Santos, Ana Serra,corresponding author Maria Matos, Luis Jesus, Ana S. Tavares, Ana Almeida,corresponding author Ceu Leit?o, Edna Varandas, Renato Abreu, Fernando Bellem, In?s A. Trindade, Claudia Ferreira, Jose Pinto-Gouveia, Joana Marta-Sim?es,corresponding author Odete Amaral,corresponding author Cristiana Miranda, Pedro Guimar?es, Rodrigo Goncalves, Nelio Veiga, Carlos Pereira, Tania C. Fleig,corresponding author Elisabete A. San-Martin, Cassia L. Goulart, Paloma B. Schneiders, Natacha F. Miranda, Lisiane L. Carvalho, Andrea G. Silva, Joana Topa,corresponding author Conceic?o Nogueira, Sofia Neves, Rita Ventura,corresponding author Cristina Nazare, Daniela Brand?o,corresponding author Alberto Freitas, oscar Ribeiro, Constanca Pa?l, Cristiana Merc?,corresponding author Marco Branco, Pedro Almeida, Daniela Nascimento, Juliana Pereira, David Catela, Helga Rafael,corresponding author Alcinda C. Reis,corresponding author Ana Mendes, Ana R. Valente, Marisa Lousada,corresponding author Diana Sousa,corresponding author Ana L. Baltazar, Mª Helena Loureiro, Ana Oliveira, Jose Aparicio, Alda Marques,corresponding author Alda Marques,corresponding author Ana Oliveira, Joana Neves, Rodrigo Ayoub, Luis Sousa,corresponding author Cristina Marques-Vieira, Sandy Severino, Helena Jose, In?s Cadorio,corresponding author Marisa Lousada, Marina Cunha,corresponding author Diogo Andrade, Ana Galhardo, Margarida Couto, Fernando Mendes,corresponding author Catia Domingues, Susann Schukg, Ana M. Abrantes, Ana C. Goncalves, Tiago Sales, Ricardo Teixo, Rita Silva, Jessica Estrela, Mafalda Laranjo, Jo?o Casalta-Lopes, Clara Rocha, Paulo C. Sim?es, Ana B. Sarmento-Ribeiro, Mª Filomena Botelho, Manuel S. Rosa, Virginia Fonseca,corresponding author Diogo Colaco, Vanessa Neves, Carlos Jesus, Camilla Hesse, Clara Rocha, Nadia Osorio, Ana Valado, Armando Caseiro, Antonio Gabriel, Lola Svensson, Fernando Mendes,corresponding author Wafa A. Siba, Cristina Pereira, Jorge Tomaz, Teresa Carvalho,corresponding author Jose Pinto-Gouveia, Marina Cunha, Diana Duarte, Nuno V. Lopes, Rui Fonseca-Pinto,corresponding author Diana Duarte, Nuno V. Lopes, Rui Fonseca-Pinto,corresponding author Anabela C. Martins, Piedade Brand?o, Laura Martins,corresponding author Margarida Cardoso, Nuno Morais,corresponding author Joana Cruz, Nuno Alves,corresponding author Paula Faria, Artur Mateus, Pedro Morouco, Nuno Alves,corresponding author Nelson Ferreira, Artur Mateus, Paula Faria, Pedro Morouco, Isabel Malheiro,corresponding author Filomena Gaspar, Luisa Barros, Pedro Parreira,corresponding author Andreia Cardoso, Lisete Monico, Carla Carvalho, Albino Lopes, Anabela Salgueiro-Oliveira, Aderito Seixas,corresponding author Valter Soares, Tiago Dias, Ricardo Vardasca, Joaquim Gabriel, Sandra Rodrigues, Hugo Paredes,corresponding author Arsenio Reis, Sara Marinho, Vitor Filipe, Jorge Lains, Jo?o Barroso, Carolina Da Motta,corresponding author Celia B. Carvalho, Jose Pinto-Gouveia, Ermelindo Peixoto, Ana A. Gomes,corresponding author Vanessa Costa, Diana Couto, Daniel R. Marques, Jose A. Leit?o, Jose Tavares, Maria H. Azevedo, Carlos F. Silva, Jo?o Freitas,corresponding author Pedro Parreira, Jo?o Mar?co, Miguel A. Garcia-Gordillo,corresponding author Daniel Collado-Mateo, Gang Chen, Angelo Iezzi, Jose A. Sala, Jose A. Parraca, Narcis Gusi, Jani Sousa,corresponding author Mariana Marques, Jacinto Jardim, Anabela Pereira, Sonia Sim?es, Marina Cunha, Pedro Sardo,corresponding author Jenifer Guedes, Jo?o Lindo, Paulo Machado, Elsa Melo, Celia B. Carvalho,corresponding author Joana Benevides, Marina Sousa, Joana Cabral, Carolina Da Motta, Ana T. Pereira,corresponding author Sandra Xavier, Julieta Azevedo, Elisabete Bento, Cristiana Marques, Rosa Carvalho, Mariana Marques, Antonio Macedo, Ana M. Silva, Juliana Alves, Ana A. Gomes,corresponding author Daniel R. Marques, Mª Helena Azevedo, Carlos Silva, Ana Mendes,corresponding author Huei D. Lee, Newton Spola?r, Jefferson T. Oliva, Wu F. Chung, Rui Fonseca-Pinto, Keila Bairros,corresponding author Claudia D. Silva, Clovis A. Souza, Silvana S. Schroeder, Elsa Ara?jo, Helena Monteiro, Ricardo Costa, Sara S. Dias,corresponding author Jorge Torgal, Carolina G. Henriques,corresponding author Luisa Santos, Elisa F. Caceiro, Sonia A. Ramalho, Rita Oliveira,corresponding author Vera Afreixo, Jo?o Santos, Priscilla Mota, Agostinho Cruz, Francisco Pimentel, Rita Marques,corresponding author Mª Anjos Dixe, Ana Querido, Patricia Sousa, Joana Benevides,corresponding author Carolina Da Motta, Marina Sousa, Suzana N. Caldeira, Celia B. Carvalho, Ana Querido,corresponding author Catarina Tomas, Daniel Carvalho, Jo?o Gomes, Marina Cordeiro, Joyce O. Costa,corresponding author Frederico C. Valim, Ligia C. Ribeiro, Zaida Charepe,corresponding author Ana Querido, Mª Henriqueta Figueiredo, Priscila S. Aquino,corresponding author Samila G. Ribeiro, Ana B. Pinheiro, Paula A. Lessa, Mirna F. Oliveira, Luisa S. Brito, italo N. Pinto, Alessandra S. Furtado, Regia B. Castro, Caroline Q. Aquino, Eveliny S. Martins, Ana B Pinheiro, Priscila S. Aquino,corresponding author Lara L. Oliveira, Patricia C. Pinheiro, Caroline R. Sousa, Vivien A. Freitas, Tatiane M. Silva, Adman S. Lima, Caroline Q. Aquino, Karizia V. Andrade, Camila A. Oliveira, Eglidia F. Vidal, Ana Ganho-avila,corresponding author Mariana Moura-Ramos, oscar Goncalves, Jorge Almeida, Armando Silva,corresponding author Irma Brito, Jo?o Amado, Antonio Rodrigo, Sofia Santos,corresponding author Fernando Gomes, Marlene C. Rosa,corresponding author Silvana F. Marques, Sara Luis,corresponding author Luis Cavalheiro, Pedro Ferreira, Rui Goncalves, Rui S. Lopes,corresponding author Luis Cavalheiro, Pedro Ferreira, Rui Goncalves, Bruno H. Fiorin,corresponding author Marina S. Santos, Edmar S. Oliveira, Rita L. Moreira, Elizabete A. Oliveira, Braulio L. Filho, Lara Palmeira,corresponding author Teresa Garcia, Jose Pinto-Gouveia, Marina Cunha, Sara Cardoso, Lara Palmeira,corresponding author Marina Cunha, Jose Pinto-Gouveia, Joana Marta-Sim?es,corresponding author Ana L. Mendes, In?s A. Trindade, Sara Oliveira, Claudia Ferreira, Ana L. Mendes,corresponding author Joana Marta-Sim?es, In?s A. Trindade, Claudia Ferreira, Filipe Nave,corresponding author Mariana Campos, Iris Gaud?ncio, Fernando Martins, Lino Ferreira, Nuno Lopes, Rui Fonseca-Pinto,corresponding author Rogerio Rodrigues,corresponding author Zaida Azeredo, Coralia Vicente, Joana Silva,corresponding author Patricia Sousa, Rita Marques, Isabel Mendes,corresponding author Rogerio Rodrigues, Zaida Azeredo, Coralia Vicente, Ricardo Vardasca,corresponding author Ana R. Marques, Aderito Seixas, Rui Carvalho, Joaquim Gabriel, Paulo P. Ferreira,corresponding author Michelle T. Oliveira, Anderson R. Sousa, Ana S. Maia, Sebasti?o T. Oliveira, Pablo O. Costa, Maiza M. Silva, Cristina Arreguy-Sena,corresponding author Nathalia Alvarenga-Martins, Paulo F. Pinto, Denize C. Oliveira, Pedro D. Parreira, Ant?nio T. Gomes, Luciene M. Braga, Odete Ara?jo,corresponding author Isabel Lage, Jose Cabrita, Laetitia Teixeira, Rita Marques,corresponding author Mª Anjos Dixe, Ana Querido, Patricia Sousa, Sara Silva,corresponding author Eugenio Cordeiro, Jo?o Pimentel, Vera Ferro-Lebres,corresponding author Juliana A. Souza, Mariline Tavares, Mª Anjos Dixe, Pedro Sousa,corresponding author Rui Passadouro, Teresa Peralta, Carlos Ferreira, Georgina Lourenco, Jo?o Serrano,corresponding author Jo?o Petrica, Rui Paulo, Samuel Honorio, Pedro Mendes, Alexandra Sim?es, Lucinda Carvalho, Alexandre Pereira, Sara Silva,corresponding author Paulino Sousa, Jose M. Padilha, Daniela Figueiredo,corresponding author Carolina Valente, Alda Marques, Patricia Ribas,corresponding author Joana Sousa, Frederico Brand?o, Cesar Sousa, Matilde Martins, Patricia Sousa,corresponding author Rita Marques, Francisco Mendes, Rosina Fernandes,corresponding author Emilia Martins, Catia Magalh?es, Patricia Ara?jo, Carla Grande,corresponding author Mª Augusta Mata, Juan G. Vieitez, Bruna Bianchini, Nazare Nazario,corresponding author Jo?o G. Filho, Marcia Kretzer, Tania Costa,corresponding author Armando Almeida, Gabriel Baffour, Armando Almeida,corresponding author Tania Costa, Gabriel Baffour, Zaida Azeredo,corresponding author Carlos Laranjeira, Magda Guerra, Ana P. Barbeiro, Regina Ferreira,corresponding author Sara Lopes,corresponding author Liliana Nunes, Ana Mendes, Julian Martins,corresponding author Dulcineia Schneider, Marcia Kretzer, Flavio Magajewski, Celia Soares,corresponding author Antonio Marques, Marco Batista,corresponding author Ruth J. Castuera, Helena Mesquita, Antonio Faustino, Jorge Santos, Samuel Honorio, Betina P. Vizzotto,corresponding author Leticia Frigo, Hedioneia F. Pivetta, Dolores Sardo,corresponding author Cristina Martins,corresponding author Wilson Abreu, Mª Ceu Figueiredo, Marco Batista,corresponding author Ruth Jimenez-Castuera, Jo?o Petrica, Jo?o Serrano, Samuel Honorio, Rui Paulo, Pedro Mendes, Patricia Sousa,corresponding author Rita Marques, Antonio Faustino,corresponding author Paulo Silveira, Jo?o Serrano, Rui Paulo, Pedro Mendes, Samuel Honorio, Catarina Oliveira,corresponding author Fernanda Bastos, In?s Cruz, Claudia K. Rodriguez, Marcia R. Kretzer, Nazare O. Nazario,corresponding author Pedro Cruz,corresponding author Daniela C. Vaz,corresponding author Rui B. Ruben, Francisco Avelelas, Susana Silva, Mª Jorge Campos, Maria Almeida,corresponding author Liliana Goncalves, Ligia Antunes, Pedro Sardo,corresponding author Jenifer Guedes, Jo?o Sim?es, Paulo Machado, Elsa Melo, Susana Cardoso,corresponding author Osvaldo Santos, Carla Nunes, Isabel Loureiro, Flavia Santos, Gilberto Alves, Claudia Soar,corresponding author Teresa O. Marsi, Ernestina Silva,corresponding author Dora Pedrosa, Andrea Leca, Daniel Silva, Ana Galv?o,corresponding author Maria Gomes,corresponding author Paula Fernandes, Ana None, Jaime Combad?o,corresponding author Catia Ramalhete, Paulo Figueiredo, Patricia Caeiro, Karine C. Fontana, Josimari T. Lacerda,corresponding author Patricia O. Machado, Raphaelle Borges,corresponding author Flavio Barbosa, Dayse Sa, Germana Brunhoso, Graca Aparicio,corresponding author Amancio Carvalho, Ana P. Garcia,corresponding author Paula O. Fernandes, Adriana Santos, Nelio Veiga,corresponding author Carina Bras, In?s Carvalho, Joana Batalha, Margarida Gloria, Filipa Bexiga, In?s Coelho, Odete Amaral, Carlos Pereira, Claudia Pinho,corresponding author# Nilson Paraiso,# Ana I. Oliveira, Cristov?o F. Lima, Alberto P. Dias, Pedro Silva, Mario Espada, Mario Marques, Ana Pereira,corresponding author Ana Mª Pereira,corresponding author Mª Veiga-Branco, Filomena Pereira, Maria Ribeiro, Vera Lima, Ana I. Oliveira, Claudia Pinho, Graca Cruz, Rita F. Oliveira, Luisa Barreiros, Fernando Moreira,corresponding author Ana Camarneiro,corresponding author Mª Helena Loureiro, Margarida Silva, Catarina Duarte, angelo Jesus,corresponding author Agostinho Cruz, Maria Mota,corresponding author Sandra Novais, Paulo Nogueira, Ana Pereira, Lara Carneiro, Paulo V. Jo?o, Teresa Maneca Lima,corresponding author Anabela Salgueiro-Oliveira,corresponding author Marina Vaquinhas, Pedro Parreira, Rosa Melo, Jo?o Graveto, Amelia Castilho, Jose H. Gomes, Maria S. Medina,corresponding author Valeriana G. Blanco, Osvaldo Santos,corresponding author Elisa Lopes, Ana Virgolino, Alexandra Dinis, Sara Ambrosio, In?s Almeida, Tatiana Marques, Mª Jo?o Heitor, Miguel A. Garcia-Gordillo,corresponding author Daniel Collado-Mateo, Pedro R. Olivares, Jose A. Parraca, Jose A. Sala, Amelia Castilho,corresponding author Jo?o Graveto, Pedro Parreira, Anabela Oliveira, Jose H. Gomes, Rosa Melo, Marina Vaquinhas, Monica Cheio, Agostinho Cruz, Olivia R. Pereira,corresponding author Sara Pinto, Adriana Oliveira, M. Conceic?o Manso, Carla Sousa, Ana F. Vinha,corresponding author Mª Manuela Machado,corresponding author Margarida Vieira, Beatriz Fernandes,corresponding author Teresa Tomas, Diogo Quirino, Gustavo Desouzart,corresponding author Rui Matos, Magali Bordini, Pedro Mouroco, Ana R. Matos,corresponding author Mauro Serapioni, Teresa Guimar?es,corresponding author Virginia Fonseca, Andre Costa, Jo?o Ribeiro, Jo?o Lobato, Inmaculada Z. Martin,corresponding author Anita Bj?rklund, Aida I. Tavares,corresponding author Pedro Ferreira, Rui Passadouro, Sonia Morgado,corresponding author Nuno Tavares,corresponding author Jo?o Valente, Anabela C. Martins, Patricia Ara?jo, Rosina Fernandes,corresponding author Francisco Mendes, Catia Magalh?es, Emilia Martins, Pedro Mendes,corresponding author Rui Paulo, Antonio Faustino, Helena Mesquita, Samuel Honorio, Marco Batista, Josimari T. Lacerda,corresponding author Angela B. Ortiga, Mª Cristina Calvo, S?nia Natal, Marta Pereira,corresponding author Manuela Ferreira,corresponding author Ana R. Prata, Paula Nelas, Jo?o Duarte, Juliana Carneiro, Ana I. Oliveira, Claudia Pinho, Cristina Couto, Rita F. Oliveira, Fernando Moreira,corresponding author Ana S. Maia,corresponding author Michelle T. Oliveira, Anderson R. Sousa, Paulo P. Ferreira, Gessica M. Souza, Livia F. Almada, Milena A. Conceic?o, Eujcely C. Santiago, Sandra Rodrigues,corresponding author Gabriela Domingues, Irina Ferreira, Luis Faria, Aderito Seixas, Ana R. Costa, angelo Jesus,corresponding author Americo Cardoso, Alexandra Meireles, Armanda Colaco, Agostinho Cruz, Viviane L. Vieira,corresponding author Kellem R. Vincha, Ana Mª Cervato-Mancuso, Melissa Faria,corresponding author Claudia Reis, Marco P. Cova,corresponding author Rita T. Ascenso, Henrique A. Almeida, Eunice G. Oliveira, Miguel Santana, Rafael Pereira, Eunice G. Oliveira, Henrique A. Almeida, Rita T. Ascenso,corresponding author Rita Jesus, Rodrigo Tapadas, Carolina Tim-Tim, Catarina Cezanne, Matilde Lagoa, Sara S. Dias,corresponding author Jorge Torgal, Jo?o Lopes, Henrique Almeida, Sandra Amado, Luis Carr?o,corresponding author Madalena Cunha,corresponding author Luis Saboga-Nunes, Carlos Albuquerque, Oliverio Ribeiro, Suzete Oliveira,corresponding author Mª Carminda Morais, Emilia Martins,corresponding author Francisco Men

Publish date

2016;

PMID

27473500

Abstract

Background
The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern in Staphylococcus aureus KACC 10778, S. aureus ATCC 15564, and S. aureus CCARM 3080 exposed to sublethal concentrations of levofloxacin and oxacillin.

Results
The decreased MICs were observed in S. aureus KACC and S. aureus ATCC when exposed to levofloxacin and oxacillin, while and S. aureus CCARM remained resistance to streptomycin (512 μg/mL) in the presence of levofloxacin and imipenem (>512 μg/mL) in the presence of oxacillin. The considerable increase in extracellular and membrane-bound β-lactamase activities was observed in S. aureus ATCC exposed to oxacillin (>26 μmol/min/mL). The antibiotic susceptibility of all strains exposed to EPIs (CCCP and PAβN) varied depending on the classes of antibiotics. The relative expression levels of adhesion-related genes (clfA, clfB, fnbA, fnnB, and icaD), efflux-related genes (norB, norC, and qacA/B), and enterotoxin gene (sec) were increased more than 5-fold in S. aureus CCARM. The eno and qacA/B genes were highly overexpressed by more than 12- and 9-folds, respectively, in S. aureus CCARM exposed to levofloxacin. The antibiotic susceptibility, lactamase activity, biofilm-forming ability, efflux activity, and gene expression pattern varied with the intrinsic antibiotic resistance of S. aureus KACC, S. aureus ATCC, and S. aureus CCARM exposed to levofloxacin and oxacillin.

Conclusions
This study would provide useful information for better understating of combination therapy related to antibiotic resistance mechanisms and open the door for designing effective antibiotic treatment protocols to prevent excessive use of antibiotics in clinical practice.

Electronic supplementary material
The online version of this article (doi:10.1186/s12866-016-0791-7) contains supplementary material, which is available to authorized users.

KEYWORDS

Lactamase, Biofilm, Efflux pump, Antibiotic resistance, Staphylococcus aureus, Gene expression, Levofloxacin, Oxacillin

Title

Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to subinhibitory levels of oxacillin and levofloxacin

Author

Ara Jo1 and Juhee Ahncorresponding author1,2

Publish date

2016;

PMID

31238530

Abstract

DUSP6 is a dual-specificity phosphatase (DUSP) involved in breast cancer progression, recurrence, and metastasis. DUSP6 is predominantly cytoplasmic in HER2+ primary breast cancer cells, but the expression and subcellular localization of DUSPs, especially DUSP6, in HER2-positive circulating tumor cells (CTCs) is unknown. Here we used the DEPArray system to identify and isolate CTCs from metastatic triple negative breast cancer (TNBC) patients and performed single-cell NanoString analysis to quantify cancer pathway gene expression in HER2-positive and HER2-negative CTC populations. All TNBC patients contained HER2-positive CTCs. HER2-positive CTCs were associated with increased ERK1/ERK2 expression, which are direct DUSP6 targets. DUSP6 protein expression was predominantly nuclear in breast CTCs and the brain metastases but not pleura or lung metastases of TNBC patients. Therefore, nuclear DUSP6 may play a role in the association with cancer spreading in TNBC patients, including brain metastasis.

KEYWORDS

circulating tumor cells (CTCs), DEPArray, dual-specificity phosphatase, HER2, brain metastasis, single cell analysis, triple-negative breast cancer (TNBC)

Title

Nuclear-Biased DUSP6 Expression is Associated with Cancer Spreading Including Brain Metastasis in Triple-Negative Breast Cancer

Author

Fan Wu,1 Robert D. McCuaig,1 Christopher R. Sutton,1 Abel H. Y. Tan,1 Yoshni Jeelall,1 Elaine G. Bean,2 Jin Dai,1 Thiru Prasanna,1,3 Jacob Batham,1 Laeeq Malik,3,4 Desmond Yip,3,4 Jane E. Dahlstrom,2,4 and Sudha Rao1,*

Publish date

2019 Jun;