Soluble to 45 mg/mL (199.85 mM) in DMSO
298.0±40.0 °C at 760 mmHg
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An accelerated solvent extraction coupled with gas chromatography-tandem mass spectrometry (ASE-GC-MS/MS) method for detecting dinitolmide residue and its metabolite (3-amino-2-methyl-5-nitrobenzamide, 3-ANOT) in eggs was developed and optimized. The samples were extracted using ASE with acetonitrile as the extractant and were purified by passage through a neutral alumina solid-phase extraction column. Then, the samples were analyzed using the GC-MS/MS method. The optimized method parameters were validated according to the requirements set forth by the European Union and the Food and Drug Administration. The average recoveries of dinitolmide and 3-ANOT from eggs (egg white, egg yolk, and whole egg) at the limit of quantification (LOQ), 0.5 maximum residue limit (MRL), 1 MRL, and 2 MRL were 82.74% to 87.49%, the relative standard deviations (RSDs) were less than 4.63%, and the intra-day RSDs and the inter-day RSDs were 2.96% to 5.21% and 3.94% to 6.34%, respectively. The limits of detection and the LOQ were 0.8 to 2.8 μg/kg and 3.0 to 10.0 μg/kg, respectively. The decision limits (CCα ) were 3001.69 to 3006.48 μg/kg, and the detection capabilities (CCβ ) were 3001.74 to 3005.22 μg/kg. Finally, the new method was successfully applied to the quantitative determination of dinitolmide and 3-ANOT in 50 commercial eggs from local supermarkets.
3-ANOT; ASE; GC-MS/MS; dinitolmide; egg.
Development of an ASE-GC-MS/MS Method for Detecting Dinitolmide and Its Metabolite 3-ANOT in Eggs
Xia Zhao 1 2 3, Bo Wang 1 2 3, Kaizhou Xie 1 2 3, Yanan Liu 1 2 3, Yangyang Zhang 1 2 3, Yajuan Wang 1 2 3, Chujun Liu 1 2 3, Yawen Guo 1 2 3, Xiaona Bu 1 2 3, Genxi Zhang 1 2 3, Tao Zhang 1 2 3, Guojun Dai 1 2 3
I R Cameron, D Spackman
1982 Sep 25
The objective of the present study was to investigate the comparative efficacy of recommended dose of selected anticoccidial drugs Salinomycin and Dinitolmide, while Cocciban at three dose levels on the hematobiochemical, fecal parameters and histopathology of broilers. For this purpose, 420-day-old commercial male broiler chicks were randomly divided into 7 treatment groups with 10 replications of 6 birds each and reared in battery brooders up to 42 days of age. Groups were designated as uninfected unmedicated (T1), infected unmedicated (T2), Cocciban 500 g/ton and infected (T3), Cocciban 750 g/ton and infected (T4), Cocciban 1000 g/ton and infected (T5), Salinomycin 500 g/ton and infected (T6), and Dinitolmide and infected (T7). Groups T2, T3, T4, T5, T6, and T7 were experimentally infected at 21-day-old by 50,000 oocysts of Eimeria species. The mean fecal, lesion scores and oocyst per gram of feces were significantly (p < 0.05) highest in infected unmedicated group, while lowest in the herbal Cocciban 1000 g/ton group than all other infected medicated groups. The hematological studies revealed a reduction in TEC, Hb, and PCV from 0 to 5th day of P.I. in all infected groups except healthy control group. The birds of all the infected groups improved in the values of TEC, Hb, PCV, blood glucose, and total serum protein on 7th day of P.I., but, the improvement was significantly (p < 0.05) better in herbal Cocciban 1000 g/ton treated birds than all other infected groups. Whereas, the TWBC counts were raised from 0 to 7th day of P.I. in all the infected groups compared to healthy control and no significant (p < 0.05) difference was observed in between the infected groups. The histopathological changes consisting of desquamation of epithelial cells, cellular infiltration, hemorrhages, edema, fibrous tissue proliferation, and developing stages of E. tenella at various depths of cecal wall were higher in all the infected groups when compared to Cocciban 1000 g/ton group.
Broilers; Coccidiosis; Fecal and lesion scores; Hemoglobin; Histopathology.
Effect of Cocciban Herbal Coccidiostats on Hematobiochemical, Fecal Parameters and Cecal Histopathology of Broiler Chicken
Srinivasu B 1, V Chinni Preetam 2, Gurram Srinivas 3, D Srinivas Rao 4