White crystalline powder
(3S)-3-(4-Hydroxyphenyl)chroman-7-ol/(3S)-3-(4-hydroxyphenyl)-3,4-dihydro-2H-chromen-7-ol/Equol/(3S)-3-(4-Hydroxyphenyl)-7-chromanol/(S)-Equol/(-)-Equol/4',7-DIHYDROXYISOFLAVAN/(S)-3-(4-Hydroxyphenyl)chroman-7-ol/R,S-EQUOL/7,4'-ISOFLAVANDIOL/EQUOL, (-)-/2H-1-Benzopyran-7-ol, 3,4-dihydro-3-(4-hydroxyphenyl)-, (3S)-/4',7-ISOFLAVANDIOL/(-)-(S)-Equol
Soluble in DMSO > 10 mM
441.7±45.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:531-95-3) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
“Isoformononetin (methoxy isoflavone) is a potent osteogenic isoflavone abundantly present in Butea monosperma, Pisum sativum, Mung bean, Machaerium villosum, Medicago sativa, and Glycine max. In the current study, an LC-ESI-MS/MS method for the simultaneous evaluation of isoformononetin (IFN), daidzein (DZN) and equol (EQL) was developed and validated in rat plasma using biochanin A as an internal standard. IFN, DZN, and EQL separation was achieved by using acetonitrile and acetic acid (0.1%) in the ratio of 90:10 (% v/v) as mobile phase under isocratic conditions at a flow rate of 0.6?mL/min on Atlantis C18 (4.6?×?250?mm, 5.0?μm) column. The achieved method was linear within the concentration range of 0.5-500?ng/mL. The method was effectively applied to investigate the permeability, protein binding estimation and pharmacokinetics studies of IFN in rats. The PAMPA permeability of IFN was found to be high at pH?4.0 and 7.0. The protein binding was found to be about 91% of IFN. The oral bioavailability of IFN was found to be poor (21.6%). IFN was found to have a moderate clearance (2.9?L/h/kg) and a large apparent volume of distribution (12.1?L/kg). The plasma half-life (t1/2) and maximum attainable concentration (Cmax) of IFN at systemic circulation was found to be 1.9?±?0.6?h and 269.3?±?0.4 after oral administration.
Copyright ? 2019. Published by Elsevier B.V.”
Bioavailability; Isoflavone; Isoformononetin; Permeability; Pharmacokinetics
LC-ESI-MS/MS method for the simultaneous determination of isoformononetin, daidzein, and equol in rat plasma: Application to a preclinical pharmacokinetic study.
Raju KSR1, Rashid M2, Gundeti M2, Taneja I3, Malik MY2, Singh SK4, Chaturvedi S4, Challagundla M2, Singh SP5, Gayen JR2, Wahajuddin M6.
2019 Oct 15
To investigate the effect of racemic equol and equol enantiomers on the proliferation of colorectal cancer HCT-15 cell and the potential mechanism.
3-( 4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide( MTT) assay was used to detect the effect of different concentrations of racemic equol and equol enantiomers( 0, 0. 5, 1, 5 and 10 μmol/L) on the proliferation of colorectal cancer HCT-15 cell. Western blot was used to detect the expression of estrogen receptor( ER)and nuclear factor 2 related factor 2( Nrf2).
Racemic equol and( R) equol inhibited the proliferation of HCT-15 cell in a dose-dependent manner, whereas( S) equol had no effect on the proliferation of HCT-15 cell. Racemic equol increased the expression of ERβ and Nrf2, while( R) equol increased the expression of Nrf2.
Racemic equol can inhibit the proliferation of HCT-15 cell through estrogenic and antioxidative activity. R equol can inhibit the proliferation of HCT-15 cell through antioxidative activity, while( S) equol has no effect on the proliferation of HCT-15 cell.”
HCT-15 cell; colorectal cancer; equol; equol enantiomers; estrogen receptor
[Effects of equol on proliferation of colorectal cancer HCT-15 cell].
Zou Y1, Wang Y1, Cai Y1, Ma D1.
We previously found that daidzein decreased food intake in female rats. To understand the mechanism of anorectic action of dietary daidzein, it is necessary to determine distributions of daidzein and S-equol, a metabolite of intestinal bacterial conversion from daidzein, in the body. In the present study, we measured the concentrations of daidzein and S-equol in serum and bile in sham-operated and ovariectomized female rats fed a diet containing 150 mg/kg daidzein for 7 days. Dietary daidzein increased serum and bile concentrations of S-equol to far higher levels than those of daidzein. S-equol concentration was more than several hundred fold-higher in bile than in serum, regardless of ovariectomy. Moreover, to investigate whether accumulation of S-equol is facilitated by efficient enterohepatic circulation during continuous intake of daidzein and S-equol, female rats were fed diet containing daidzein or S-equol (both 150 mg/kg), or control diet for 1, 2, 3, or 5 days. Dietary daidzein significantly increased serum and bile concentrations of S-equol in a time-dependent manner, but not those of daidzein. These results indicated that substantial proportion of dietary daidzein was converted to S-equol, which underwent efficient enterohepatic circulation and predominantly accumulated there
Dietary daidzein induces accumulation of S-equol in enterohepatic circulation to far higher levels than that of daidzein in female rats with and without ovariectomy.
Fujitani M1, Mizushige T2, Bhattarai K1,3, Adhikari S3, Ishikawa J4, Kishida T1,3.
(-)-(S)-Equol is a high affinity ligand for estrogen receptor β with a Ki of 0.73 nM.