Off-white crystalline powder
Astrocladus coniferus,Ganoderma duropora,Lentinula edodes,Peristrophe japonica,Shiraia bambusicola
Ergosterol/(24R)-Ergosta-5,7,22-trien-3b-ol/(3S,9S,10R,13R,14R,17R)-17-[(2R,3E,5R)-5,6-Dimethyl-3-hepten-2-yl]-10,13-dimethyl-2,3,4,9,10,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol/PROVITAMIN D2/ERGOSTERINE/(22E)-Ergosta-5,7,22-trien-3β-ol/ERGOSTERIN/Ergosta-5,7,22-trien-3β-ol/PROVITAMINE D2/(3β)-Ergosta-5,7,22-trien-3-ol/(3b,22E)-Ergosta-5,7,22-trien-3-ol/Ergosta-5,7,22-trien-3-ol, (3β,22E)-/(3β,2E)-Ergosta-5,7,22-trien-3-ol/provitamind/(3β,22E)-Ergosta-5,7,22-trien-3-ol/Ergosta-5:6,7:8,22:23-trien-3-ol
501.5±39.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:57-87-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Background: This is the first report demonstrating the antibiotic-modifying activity of cholecalciferol.
In this study, cholecalciferol was evaluated against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli.
The antibacterial and modulatory effects of cholecalciferol, ergosterol, and cholesterol (8-512 μg/mL) were evaluated by microdilution assay against multiresistant bacterial strains.
Cholecalciferol, when combined with aminoglycosides, was more effective against P. aeruginosa, reducing the concentration of amikacin and gentamicin necessary to inhibit bacterial growth from 156.25 to 39.06 μg/mL and from 39.06 to 9.76 μg/mL, respectively. It is possible that cholecalciferol, due to its lipid-soluble nature, had a lipophilic interaction with the cell membrane, enhancing antibiotic uptake. Cholesterol and ergosterol were used to see if the mechanism of action of cholecalciferol was similar to that of these lipid compounds. Ergosterol and cholesterol increased aminoglycoside activity, where the effect was greater with higher subinhibitory concentration of sterol.
There is no reported study on the use of cholesterol and ergosterol as modulators of antibiotics or any other drug, making this the first study in this area highlighting the interaction between cholesterol, ergosterol, and cholecalciferol with regard to modifying aminoglycoside activity.
Cholecalciferol; aminoglycosides; antibiotic-modifying; bacterial resistance; lipid solubility
Cholecalciferol, Ergosterol, and Cholesterol Enhance the Antibiotic Activity of Drugs.
Andrade JC1, Morais Braga MFB1, Guedes GMM1, Tintino SR1, Freitas MA1, Quintans LJ1, Jr2, Menezes IRA1, Coutinho HDM1.
In the field of natural products, dereplication of complex mixtures hasbecomes usual practice to annotate known compounds and avoid their re-isolation. To this purpose, many groups rely on liquid chromatography coupled to high resolution mass spectrometry to deduce molecular formulae of compounds allowing comparison with public or in-house databases. Electrospray ionisation is usually considered as the method of choice to investigate a large panel of compounds but, in some cases, it may lead to unusual results as described in the following article for ergosterol.
Ergosterol and other fungal sterols in methanolic solution were analysed using different chromatographic gradients by HPLC/MS on both IT-TOFMS and Orbitrap-MS instruments fitted with an ESI source. Further flow injection analyses were performed to investigate the influence of the solvent composition. MS/MS fragmentation data were acquired to annotate the different ions observed.
Contrary to other fungal sterols, ergosterol was found to be highly sensitive to oxidation during electrospray ionisation. Putative structures were proposed based on MS/MS studies and known oxidation mechanisms of ergosterol by reactive oxygen species (ROS) that could be formed in the ESI process. The proportion of acetonitrile in the eluent was found to influence this in-source oxidation, with increased proportion of oxidized sodium adducts with higher proportions of acetonitrile.
While ergosterol is a major sterol found in fungi, this study investigates its ionisation by electrospray for the first time. The results reported here will help further detection and annotation of this compound in fungal extracts after HPLC/ESI-MS analyses.
This article is protected by copyright. All rights reserved.
Detection of ergosterol by liquid chromatography/electrospray mass spectrometry - investigation of unusual in source reactions.
Ory L1, Gentil E1,2, Kumla D3, Kijjoa A3, Nazih EH1, Roullier C1,2.
2020 Mar 10
Androgen-dependent LNCaP and androgen-independent DU-145 cells, were treated with different concentrations of ergosterol (15 µM and 25 µM) and its respective cell viability was measured by MTT bioassay. While ergosterol showed an antiproliferative effect on LNCaP, on DU-145 promoted cell proliferation. This differential effect suggests that the effect of ergosterolmight be related to its ability to act as an Androgen Receptor ligand. In silico Molecular Dynamics simulations were performed to analyze the interaction mechanism between androgen receptor and ergosterol, in comparison with natural ligands, 5α-dihydrotestosterone and testosterone. Our model suggests that the binding of androgen receptor with ergosterol is thermodinamically feasible, which is concordant with our experimental results.
Androgen receptor; DU-145; LNCaP; MTT bioassay; ergosterol; molecular dynamics simulations
Ergosterol exerts a differential effect on AR-dependent LNCaP and AR-independent DU-145 cancer cells.
MuNoz-Fonseca MB1, Vidal-Limon A2, Fernandez-Pomares C3, Rojas-Duran F3, Hernandez-Aguilar ME3, Espinoza C4, Trigos A4, Suarez-Medellin J1.
2020 Mar 9