Catalogue Number
BF-E3009
Analysis Method
HPLC,NMR,MS
Specification
98%
Storage
2-8°C
Molecular Weight
178.14
Appearance
Yellow crystalline powder
Botanical Source
peel of Aesculus hippocastanum L.
Structure Type
Phenylpropanoids
Category
Standards;Natural Pytochemical;API
SMILES
C1=CC(=O)OC2=CC(=C(C=C21)O)O
Synonyms
Aesculetin/Cryptotanshinone/ESCULETOL/CICHERINGENIN/Cichorigenin/Asculetine/Esculatin/6,7-Dihydroxycoumarin/Esculin aglycon/6,7-Dihydroxy-2H-chromen-2-one/esculetin/2H-1-Benzopyran-2-one, 6,7-dihydroxy-/6,7-dihydroxy-2H-1-benzopyran-2-one/Coumarin, 6,7-dihydroxy-
IUPAC Name
6,7-dihydroxychromen-2-one
Density
1.6±0.1 g/cm3
Solubility
Methanol; Ethanol
Flash Point
201.5±22.2 °C
Boiling Point
469.7±45.0 °C at 760 mmHg
Melting Point
271-273 °C(lit.)
InChl
InChl Key
WGK Germany
RID/ADR
HS Code Reference
2932200000
Personal Projective Equipment
Correct Usage
For Reference Standard and R&D, Not for Human Use Directly.
Meta Tag
provides coniferyl ferulate(CAS#:305-01-1) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
MSDS
29108761
Introduction: Esculetin was identified to inhibit cell proliferation and induce apoptosis or cell cycle arrest in several cancer cell lines. However, the effect of esculetin on lung cancer remains elusive.
Methods: The anti-proliferative role of esculetin in murine Lewis lung carcinoma (LLC) cells was evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and colony formation assays. BALB/c mice were subcutaneously injected with LLC cells to investigate the inhibitory effect of esculetin on the growth of lung cancer xenograft. Invasive ability was detected in esculetin treated and untreated LLC cells by transwell assay. The association between esculetin and Wnt/β-catenin signaling, as well as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), was confirmed by testing the expression of c-myc, Cyclin D1 and NF-κB using Western blot.
Results: Esculetin treatment in LLC cells led to significant decrease of cell proliferation in a time- and dose-dependent manner. After injection of LLC cells into mice, reduced size and weight of tumors were observed in esculetin treated mice compared to untreated mice. However, no difference in cell invasion was observed between the treated and untreated LLC cells. Notably decreased expression of c-myc, Cyclin D1 and NF-κB were observed in LLC cells with esculetin treatment compared to untreated cells.
Conclusion: Esculetin plays an inhibitory role in the growth of lung cancer by down-regulating c-myc, Cyclin D1 and NF-κB.
C-myc; Ciclina D1; Cyclin D1; Cancer de pulmon; Esculetin; Esculetina; Factor nuclear potenciador de las cadenas ligeras kappa de celulas B activadas (NF-κB); Lung cancer; Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB).
Esculetin Attenuates the Growth of Lung Cancer by Downregulating Wnt Targeted Genes and Suppressing NF-κB
Xiangyun Zhu 1 , Jiaping Gu 1 , Hongxian Qian 2
2018 Mar
28177662
Esculetin (6,7-dihydroxycoumarin) is a coumarin derivative extracted from natural plants and has been reported to have anticancer activity. However, the mechanism by which esculetin prevents human hepatic cancer cell growth is still largely unknown. In this study, we investigated the effect of esculetin on human hepatocellular carcinoma (HCC) SMMC-7721 cells and explored the cell signal mechanism. Our data indicated that esculetin induced apoptosis in SMMC-7721 cells, which were supported by DAPI staining and Annexin V/PI staining. Meanwhile, esculetin increased the activities of caspase-3 and caspase-9, promoted bax expression, decreased bcl-2 expression, and triggered collapse of mitochondrial membrane potential, and increased cytochrome c release from mitochondria. In addition, the inactivation of IGF-1, PI3K, and Akt was observed after esculetin administration. Furthermore, pretreatment with IGF-1 before esculetin administration abrogated the pro-apoptotic effects of esculetin, while PI3K inhibitor increased the pro-apoptotic effects of esculetin. These results indicated that esculetin induced the apoptosis of SMMC-7721 cells through IGF-1/PI3K/Akt-regulated mitochondrial dysfunction.
C-myc; Ciclina D1; Cyclin D1; Cancer de pulmon; Esculetin; Esculetina; Factor nuclear potenciador de las cadenas ligeras kappa de celulas B activadas (NF-κB); Lung cancer; Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB).
Esculetin Induces Apoptosis of SMMC-7721 Cells Through IGF-1/PI3K/Akt-mediated Mitochondrial Pathways
Juan Li 1 , Shuang Li 2 , Xiuli Wang 1 , Hongxin Wang 3
2017 Jul
29705703
Esculetin (6,7-dihydroxycoumarin), a natural coumarin compound extracted from natural plants, was reported to be involved in ischemia/reperfusion (I/R) injury. However, the role of esculetin in myocardial I/R injury remains unclear. This study was designed to investigate the protective effects of esculetin on cardiomyocytes induced by hypoxia/reoxygenation (H/R), and explore the underlying mechanisms. Our results showed that esculetin improved the cell viability and decreased lactate dehydrogenase (LDH) release in H/R-stimulated H9c2 cells. In addition, esculetin significantly suppressed oxidative stress and apoptosis in H9c2 cells exposed to H/R treatment. Exploration of the underlying mechanisms of its action indicated that esculetin enhanced the activation of JAK2/STAT3 pathway in H/R-stimulated H9c2 cells. Taken together, these findings indicated that esculetin inhibits oxidative stress and apoptosis in H9c2 cardiomyocytes following H/R injury through the activation of JAK2/STAT3 pathway.
Apoptosis; Esculetin; Myocardial I/R injury; Oxidative stress.
Esculetin Inhibits Oxidative Stress and Apoptosis in H9c2 Cardiomyocytes Following Hypoxia/Reoxygenation Injury
Ya He 1 , Chen Li 2 , Qiaoya Ma 2 , Songsheng Chen 2
2018 Jun 18
Description :
Esculetin is an active ingredient extracted mainly from the bark of Fraxinus rhynchophylla. Esculetin inhibits platelet-derived growth factor (PDGF)-induced airway smooth muscle cells (ASMCs) phenotype switching through inhibition of PI3K/Akt pathway. Esculetin has antioxidant, antiinflammatory, and antitumor activities[1].
