This product is isolated and purified from the roots of Gardneria multiflora Makino
(12S,13S,16Z,17S)-3,4,6-Trimethoxy-16-(2-methoxyethylidene)-10-oxa-8,14-diazahexacyclo[126.96.36.199.0.0.0]icosa-2,4,6,8-tetraene/8,11,12a-Ethanylylidene-7H-pyrido[2',3':5,6]oxepino[2,3-b]indole, 7a,8,9,10,11a,12-hexahydro-1,2,4-trimethoxy-9-(2-methoxyethylidene)-, (7aS,8S,9Z,11aS)-/(5β,19E)-9,10,12,18-Tetramethoxy-1,2-didehydro-2,17-epoxy-5,16-cyclocorynox-19-en/8,11,12a-Ethanylylidene-7H-pyrido[2',3':5,6]oxepino[2,3-b]indole, 7a,8,9,10,11a,12-hexahydro-1,2,4-trimethoxy-9-(2-methoxyethylidene)-, (8S,9E,11aS,12aS,13S)-
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
570.3±60.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:34274-91-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
As a novel monoterpenoid indole alkaloid, gardneramine has been confirmed to possess excellent nervous depressive effects. However, there have been no reports about the measurement of gardneramine in vitro and in vivo. The motivation of this study was to establish and validate a specific, sensitive, and robust analytical method based on UHPLC-MS/MS for quantification of gardneramine in rat plasma and various tissues after intravenous administration. The analyte was extracted from plasma and tissue samples by protein precipitation with methanol using theophylline as an internal standard (I.S.). The analytes were separated on an Agilent ZORBAX Eclipse Plus C18 column using a gradient elution of acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Gardneramine and I.S. were detected and quantified using positive electrospray ionization in multiple reaction monitoring (MRM) mode with transitions of m/z 413.1→217.9 for gardneramine and m/z 181.2→124.1 for I.S.. Perfect linearity range was 1-2000 ng/mL with a correlation coefficient (r2) of ≥0.990. The lower limit of quantification (LLOQ) of 1.0 ng/mL was adequate for application to different preclinical studies. The method was successfully applied for determination of gardneramine in bio-samples.
LC-MS/MS; gardneramine; monoterpenoid indole alkaloid; pharmacokinetics; tissue distribution.
Development and Validation of a Sensitive UHPLC-MS/MS Method for the Measurement of Gardneramine in Rat Plasma and Tissues and its Application to Pharmacokinetics and Tissue Distribution Study.
Zhao N1, Tan HR2, Chen QL3, Sun Q4, Wang L5, Song Y6, Olounfeh KM7, Meng FH8.
2019 Oct 31;
The ganglion blocking site of gardneramine (GA) and hirsutine (HS) was studied in the dog urinary bladder in an in situ preparation. GA and HS selectively inhibited the DMPP-induced contraction without having an antagonistic effect on the McN-A-343-induced and acetylcholine-induced contraction. In addition, since GA and HS showed a local anesthetic action weaker than that of procaine, the effect of procaine was studied on the same preparation. Procaine inhibited the McN-A-343-induced contraction, and it slightly inhibited the DMPP-induced and acetylcholine-induced contraction. From these findings, it is concluded that GA and HS inhibited the ganglionic transmission of the dog urinary bladder and that the blockade of the nicotinic receptor played a main role.
Site of the ganglion blocking action of gardneramine and hirsutine in the dog urinary bladder in situ preparation.
Ozaki Y, Harada M.