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  • Brand : BIOFRON

  • Catalogue Number : BF-H2003

  • Specification : 98%

  • CAS number : 80154-34-3

  • Formula : C13H16O7

  • Molecular Weight : 284.26

  • PUBCHEM ID : 12896796

  • Volume : 20mg

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Catalogue Number


Analysis Method






Molecular Weight



White crystalline powder

Botanical Source

roots of Helicia erratica Hook. f.

Structure Type



Standards;Natural Pytochemical;API




2-formylphenyl beta-L-glucopyranoside/HILICIDUM/HEARTLEAFHOUTTUYNIAP.E./Benzaldehyde, 4-(β-D-glucopyranosyloxy)-/Gynostemma/4-Formylphenyl β-D-glucopyranoside/Hiliedum/HELICIDUM/HELICIDE/Benzaldehyde, 4-(β-D-allopyranosyloxy)-/Helicid/4-Formylphenyl β-D-allopyranoside/4-Formylphenylb-D-allopyranoside




1.5±0.1 g/cm3


Flash Point

215.8±23.6 °C

Boiling Point

559.9±50.0 °C at 760 mmHg

Melting Point



InChl Key

WGK Germany


HS Code Reference


Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:80154-34-3) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate




Helicid (4-formylphenyl-O-β-D-allopyranoside), an active component found in seeds from the Chinese herb Helicia nilagirica, has been reported to exert sedative, analgesic, hypnotic and antidepressant effects. The present study was designed to evaluate the antidepressant, learning and cognitive improvement effects of helicid in a chronic unpredictable mild stress (CUMS) model of depression in rats and to explore cAMP/protein kinase A (PKA)/cAMP response element-binding (CREB) signaling pathway. Sprague-Dawley rats were randomly assigned to six groups (n = 10): control; CUMS; CUMS + fluoxetine (5 mg/kg) and CUMS + helicid at 8, 16 and 32 mg/kg. All rats were subjected to 12 weeks of CUMS protocols and drug administration during the last 6 weeks of CUMS. Our results showed that helicid, at a dose of 32 mg/kg, significantly reversed decreases in body weight and sucrose consumption, increased the distance and number of crossings in the open-field test (OFT), reduced immobility times in the forced swimming test (FST) and improved spatial memory in the Morris water maze (MWM); all of these effects had been induced by CUMS paradigm. Immunohistochemistry showed that administration of helicid could promoted the proliferation of neurons in the hippocampal CA1 and dentate gyrus (DG) regions. CUMS rats treated with helicid had dramatically decreased protein levels of serotonin transporters (SERTs). In addition, CUMS resulted in a significant reduction in the expression of cAMP, PKA C-α and p-CREB, each of which were partially attenuated by helicid administration. These results indicated that helicid could improve depressive behaviors, learning and cognitive deficits and increase hippocampal neurogenesis, which may be mediated by the regulation of SERTs, activation of the cAMP/PKA/CREB signaling pathway and upregulation of p-CREB levels in hippocampal.


chronic unpredictable mild stress (CUMS); cognitive ability; depression; learning ability; serotonin transporter (SERT)


Helicid Ameliorates Learning and Cognitive Ability and Activities cAMP/PKA/CREB Signaling in Chronic Unpredictable Mild Stress Rats.


Li XY1, Qi WW2, Zhang YX1, Jiang SY1, Yang B3, Xiong L1, Tong JC1,3.

Publish date





Helicid (4-formylphenyl-β-D-allopyranoside) is a bioactive constituent of Helicid nilgirica Bedd that has been used in Chinese traditional herbal medicine to treat headache, insomnia, and depression. However, the underlying mechanisms of these effects are unclear. We have now investigated the effect of helicid on depression-related behaviors in rats exposed to chronic unpredictable mild stress (CUMS) and have also explored possible underlying mechanisms that involve neurotrophin expression. After 6 weeks isolation, body weight and sucrose preference were significantly reduced in rats with CUMS-induced depression compared with controls. The CUMS rats also showed significant inhibition of locomotory parameters in open field tests (involving behavioral assays). Helicid significantly regulated levels of corticosterone (CORT), inflammatory cytokines and 5-hydroxytryptamine (5-HT). Helicid also reversed CUMS-induced decreases of 5-HT1A receptor expression and promoted brain derived neurotrophic factor (BDNF) expression in the hippocampus The significant reversal of depressive-like behaviors by helicid is similar to that achieved by fluoxetine. The antidepressive effects are likely attributable to the promotion of hippocampal neurotrophin expression through activation of the serotonergic system. Helicid thus has potential for treating depressive disorders.

Copyright © 2018 Elsevier B.V. All rights reserved.


Antidepressant; Chronic unpredictable mild stress; Helicid; Inflammatory cytokine; Serotonergic system


Antidepressant effect of helicid in chronic unpredictable mild stress model in rats.


Tong J1, Zhou Z2, Qi W3, Jiang S4, Yang B4, Zhong Z4, Jia Y4, Li X3, Xiong L5, Nie L6.

Publish date

2019 Feb




Study on the binding properties of helicid by pepsin systematically using multi-spectroscopic techniques and molecular docking method, and these interactions comprise biological recognition at molecular level and backbone of biological significance in medicine concerned with the uses, effects, and modes of action of drugs. We investigated the mechanism of interaction between helicid and pepsin by using various spectroscopic techniques viz., fluorescence spectra, UV-Vis absorption spectra, circular dichroism (CD), 3D spectra, synchronous fluorescence spectra and molecular docking methods. The quenching mechanism associated with the helicid-pepsin interaction was determined by performing fluorescence measurements at different temperatures. From the experimental results show that helicid quenched the fluorescence intensity of pepsin via a combination of static and dynamic quenching process. The binding constants (Ka) at three temperatures (288, 298, and 308 K) were 7.940 × 107, 2.082 × 105 and 3.199 × 105 L mol-1, respectively, and the number of binding sites (n) were 1.44, 1.14, and 1.18, respectively. The n value is close to unity, which means that there is only one independent class of binding site on pepsin for helicid. Thermodynamic parameters at 298 K were calculated as follows: ΔHo (- 83.85 kJ mol-1), ΔGo (- 33.279 kJ mol-1), and ΔSo (- 169.72 J K-1 mol-1). Based on thermodynamic analysis, the interaction of helicid with pepsin is driven by enthalpy, and Van der Waals’ forces and hydrogen bonds are the main forces between helicid and pepsin. A molecular docking study further confirmed the binding mode obtained by the experimental studies. The conformational changes in the structure of pepsin was confirmed by 3D fluorescence spectra and circular dichroism.


Helicid; Molecular docking; Multi-spectroscopic techniques; Pepsin


Multi-spectroscopic studies on the interaction between traditional Chinese herb, helicid with pepsin.


Meti MD1,2, Xu Y1,3, Xie J1, Chen Y1,4, Wu Z1, Liu J5, Han Q1, He Z6, Hu Z1, Xu H7.

Publish date

2018 Dec

Description :

Helicid (Helicide, Helicidum, 4-Formylphenyl-β-D-allopyranoside) is a major constituent of Helicia nilgirica Bedd. Helicid has been used to treat psychoneurosis for its sedative-hypnotic and analgesic properties[1].