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Herpetone

$280

Brand : BIOFRON
Catalogue Number : BD-P0009
Specification : 95.0%(HPLC)
CAS number : 951677-22-8
Formula : C29H30O9
Molecular Weight : 522.54
PUBCHEM ID : 102004856
Volume : 5mg

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Catalogue Number

BD-P0009

Analysis Method

Specification

95.0%(HPLC)

Storage

-20℃

Molecular Weight

522.54

Appearance

Powder

Botanical Source

This product is isolated and purified from the fruits of Herpetospermum pedunculosum

Structure Type

Category

SMILES

COC1=CC(=CC(=C1O)CC(=O)C2=CC(=C(C=C2)O)OC)C3C4COC(C4CO3)C5=CC(=C(C=C5)O)OC

Synonyms

2-[5-[(3S,3aR,6S,6aR)-3-(4-hydroxy-3-methoxyphenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-6-yl]-2-hydroxy-3-methoxyphenyl]-1-(4-hydroxy-3-methoxyphenyl)ethanone

IUPAC Name

Density

1.3±0.1 g/cm3

Solubility

Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.

Flash Point

248.4±26.4 °C

Boiling Point

748.0±60.0 °C at 760 mmHg

Melting Point

InChl

InChl Key

NKRVXSJMQLQTTM-UGOBFYTOSA-N

WGK Germany

RID/ADR

HS Code Reference

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:951677-22-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

No Technical Documents Available For This Product.

PMID

30989979

Abstract

Herpetone( HPT) is a bioactive lignan extracted from Herpetospermum pedunculosum,which can protect liver,lower aminotransferase and inhibit hepatitis B virus. However,HPT has a poor oral bioavailability due to its poor water solubility. And there is no report about whether HPT has an anti-hepatic fibrosis activity. To improve the dissolution of HPT and study its anti-hepatic fibrosis activity and mechanism,the study group prepared herpetone nanosuspensions( HPT-NS) by the miniaturized media milling method. The formulation and process of HPT-NS were optimized by the single factor experiment. Scanning electron microscopy was used to observe morphology of HPT-NS. Dialysis method was used to study dissolution of HPT-NS in vitro. CCK8 method was used to assess the effect of HPT-NS on proliferation of the rat hepatic stellate cells( HSC-T6). Flow cytometry was used to assess the effect of HPT-NS on apoptosis and cell cycle of HSC-T6. The mean particle size of optimized HPT-NS was( 196±7) nm with a polydispersity index of 0.279±0.009.SEM showed that HPT-NS was in a regular rod shape. The cumulative dissolution rate of HPT-NS reached 93% in 18 h,and was higher than that of herpetone coarse suspensions( HPT-CS,28%). CCK8 experiment showed that the inhibition rate of HPT-NS on HSC-T6 was higher than that of HPT-CS. Flow cytometry showed that HPT-NS could block HSC-T6 cells in G2/M phase and induce apoptosis of HSC-T6 cells,with a significantly stronger effect than HPT-CS. The results revealed that HPT-NS significantly increased the in vitro dissolution of HPT,and enhanced the inhibitive effect on HSC-T6 cell proliferation by blocking cells in the G2/M phase and inducing late apoptosis.

KEYWORDS

apoptosis; cell cycle; dissolution; hepatic stellate cells; herpetone; nanosuspension.

Title

[Preparation and in vitro anti-hepatic fibrosis evaluation of herpetone nanosuspensions]

Author

Jie-Jie Zuo 1, Cheng-Ying Shen 1, Bao-de Shen 2, Yuan Liu 3, Rui-Na Zhong 1, Xiao Liu 3, Xiao-Han Wang 1, Hai-Long Yuan 3

Publish date

2019 Mar