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Holostyligone, (-)-

$807

  • Brand : BIOFRON

  • Catalogue Number : BD-P0076

  • Specification : 98.0%(HPLC)

  • CAS number : 887501-28-2

  • Formula : C21H24O5

  • Molecular Weight : 356.41

  • PUBCHEM ID : 46883472

  • Volume : 10mg

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Catalogue Number

BD-P0076

Analysis Method

HPLC,NMR,MS

Specification

98.0%(HPLC)

Storage

2-8°C

Molecular Weight

356.41

Appearance

Cryst.

Botanical Source

Structure Type

Lignans

Category

SMILES

CC1C(C(=O)C2=CC(=C(C=C2C1C3=CC(=C(C=C3)O)OC)OC)OC)C

Synonyms

(2S,3S,4R)-4-(4-hydroxy-3-methoxyphenyl)-6,7-dimethoxy-2,3-dimethyl-3,4-dihydro-2H-naphthalen-1-one

IUPAC Name

(2S,3S,4R)-4-(4-hydroxy-3-methoxyphenyl)-6,7-dimethoxy-2,3-dimethyl-3,4-dihydro-2H-naphthalen-1-one

Applications

Density

1.159±0.06 g/cm3 (20 ºC 760 Torr)

Solubility

Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.

Flash Point

175.6±23.6 °C

Boiling Point

507.0±50.0 °C at 760 mmHg

Melting Point

InChl

InChI=1S/C21H24O5/c1-11-12(2)21(23)15-10-19(26-5)18(25-4)9-14(15)20(11)13-6-7-16(22)17(8-13)24-3/h6-12,20,22H,1-5H3/t11-,12+,20-/m1/s1

InChl Key

LBJCQKYBKIAWHJ-XAAFQQQXSA-N

WGK Germany

RID/ADR

HS Code Reference

2933990000

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:887501-28-2) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

No Technical Documents Available For This Product.

PMID

26209388

Abstract

Background
Molecular assays are the gold standard methods used to diagnose viral respiratory pathogens. Pitfalls associated with this technique include limits to the number of targeted pathogens, the requirement for continuous monitoring to ensure sensitivity/specificity is maintained and the need to evolve to include emerging pathogens. Introducing target independent next generation sequencing (NGS) could resolve these issues and revolutionise respiratory viral diagnostics.

Objectives
To compare the sensitivity and specificity of target independent NGS against the current standard diagnostic test.

Study design
Diagnostic RT-PCR of clinical samples was carried out in parallel with target independent NGS. NGS sequences were analyzed to determine the proportion with viral origin and consensus sequences were used to establish viral genotypes and serotypes where applicable.

Results
89 nasopharyngeal swabs were tested. A viral pathogen was detected in 43% of samples by NGS and 54% by RT-PCR. All NGS viral detections were confirmed by RT-PCR.

Conclusions
Target independent NGS can detect viral pathogens in clinical samples. Where viruses were detected by RT-PCR alone the Ct value was higher than those detected by both assays, suggesting an NGS detection cut-off – Ct = 32. The sensitivity and specificity of NGS compared with RT-PCR was 78% and 80% respectively. This is lower than current diagnostic assays but NGS provided full genome sequences in some cases, allowing determination of viral subtype and serotype. Sequencing technology is improving rapidly and it is likely that within a short period of time sequencing depth will increase in-turn improving test sensitivity.

Abbreviations: RT-PCR, real-time polymerase chain reaction; NGS, next generation sequencing; NPS, nasopharyngeal swab; VTM, viral transport medium; HRV, human rhinovirus; IFA, influenza A; IFB, influenza B; RSV, respiratory syncytial virus; ADV, adenovirus; hMPV, human metapneumovirus; PIV-1-4, parainfluenza virus 1-4; HCoV, human coronavirus; WoSSVC, West of Scotland Specialist Virology Center; HEV, human enterovirus; Ct, cycle threshold; BLAST, basic local alignment search tool; TRT, turn-around time

KEYWORDS

Next generation sequencing, Virus detection;, Viral respiratory infection, Diagnostics

Title

The use of next generation sequencing in the diagnosis and typing of respiratory infections

Author

Fiona Thorburn,a,⁎ Susan Bennett,b Sejal Modha,a David Murdoch,c Rory Gunson,b and Pablo R. Murciaa

Publish date

2015 Aug;