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Hupehenine

$250

  • Brand : BIOFRON

  • Catalogue Number : BF-H4008

  • Specification : 98%(HPLC)

  • CAS number : 98243-57-3

  • Formula : C27H45NO2

  • Molecular Weight : 415.65

  • PUBCHEM ID : 14240934

  • Volume : 25mg

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Catalogue Number

BF-H4008

Analysis Method

HPLC,NMR,MS

Specification

98%(HPLC)

Storage

2-8°C

Molecular Weight

415.65

Appearance

White crystalline powder

Botanical Source

Fritillaria monantha

Structure Type

Alkaloids

Category

Standards;Natural Pytochemical;API

SMILES

CC1CCC2C(C3CCC4C(C3CN2C1)CC5C4CC(C6C5(CCC(C6)O)C)O)C

Synonyms

(3β,5α,6β,17β)-Cevane-3,6-diol/Cevane-3,6-diol, (3β,5α,6β,17β)-/Delavine

IUPAC Name

(1R,2S,6S,9S,10R,11R,14S,15S,17R,18S,20S,23R,24S)-6,10,23-trimethyl-4-azahexacyclo[12.11.0.02,11.04,9.015,24.018,23]pentacosane-17,20-diol

Density

1.1±0.1 g/cm3

Solubility

Methanol; Acetontrile; DMSO

Flash Point

250.4±24.6 °C

Boiling Point

538.0±35.0 °C at 760 mmHg

Melting Point

InChl

InChI=1S/C27H45NO2/c1-15-4-7-25-16(2)18-5-6-19-20(22(18)14-28(25)13-15)11-23-21(19)12-26(30)24-10-17(29)8-9-27(23,24)3/h15-26,29-30H,4-14H2,1-3H3/t15-,16+,17-,18-,19+,20+,21-,22+,23-,24+,25-,26+,27+/m0/s1

InChl Key

NEMWYOKGHGSVSC-MSSYMPDSSA-N

WGK Germany

RID/ADR

HS Code Reference

2938900000

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:98243-57-3) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

PMID

26033449

Abstract

In this work, a sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of hupehenine in rat plasma was developed and validated. After addition of imperialine as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 416.3 → 98.0 for hupehenine, and m/z 430.3 → 138.2 for IS. Calibration plots were linear throughout the range 2-2000 ng/mL for hupehenine in rat plasma. Mean recoveries of hupehenine in rat plasma ranged from 92.5 to 97.3%. Relative standard deviations of intra-day and inter-day precision were both <6%. The accuracy of the method was between 92.7 and 107.4%. The method was successfully applied to a pharmacokinetic study of hupehenine after either oral or intravenous administration. For the first time, the bioavailability of hupehenine was reported as 13.4%.

Copyright © 2015 John Wiley & Sons, Ltd.

KEYWORDS

UPLC-MS/MS; hupehenine; pharmacokinetics; rat

Title

Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study.

Author

Wen C1, Wang S2, Huang X3, Liu Z1, Lin Y1, Yang S1, Ma J3, Zhou Y2, Wang X3.

Publish date

2015 Dec

PMID

18278474

Abstract

A reverse-phase high pressure liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) has been developed for the quantitative analysis of hupehenine in the total alkaloids from Fritillaria hupehensis. Samples were analyzed on a reverse-phase column (Hypersil C-18) with a mobile phase of methanol:water:chloroform: triethylamine (85:15:1:0.6). The ELSD was set at the drift tube temperature of 68.3 degrees C and gas flow rate of 1.8 L/min. Hupehenine’s retention time was 13.7 min with an asymmetry factor of 1.2. The validity of the method has been verified with linearity, limit of detection, accuracy and precision. The logarithmic linear curve was obtained from 8.936 to 134.04 microg/mL (r=0.9993). The detection limit (S/N>3) of hupehenine was 1.79 microg/mL on the column. Intra-day RSD was 1.42% and inter-day RSD was 2.26% (3 days within a week). The average recovery of hupehenine was 101.50%, and RSD was 1.62%.

Title

Analysis of hupehenine in the total alkaloids from Fritillaria hupehensis by HPLC-ELSD.

Author

Zhang P1, Li J, Zhang G, Pi H, Zhang Y, Ruan H, Wu J.

Publish date

2008 Feb


Description :

Hupehenine, a bioactive isosteroidal alkaloid, is a main antitussive components present in most of Fritillariae Bulbus[1].