(2R,3R)-2-(2,4-Dihydroxyphenyl)-3,5,7-trihydroxy-6,8-bis(3-methyl-2-buten-1-yl)-2,3-dihydro-4H-chromen-4-one/(2R,3R)-2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxy-6,8-bis(3-methylbut-2-en-1-yl)-2,3-dihydro-4H-chromen-4-one/4H-1-Benzopyran-4-one, 2-(2,4-dihydroxyphenyl)-2,3-dihydro-3,5,7-trihydroxy-6,8-bis(3-methyl-2-buten-1-yl)-, (2R,3R)-
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
715.7±60.0 °C at 760 mmHg
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provides coniferyl ferulate(CAS#:101236-50-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Chlorination of tyrosine is a commonly known effect/consequence of myeloperoxidase activity at sites of inflammation, and detection of 3-chlorotyrosine has been used as biomarker for inflammatory diseases. However, few studies have addressed site specific chlorination in proteins, and no methods for large scale chloroproteomics studies have yet been published.
In this study, we present an optimized mass spectrometry based protocol to identify and quantify chlorinated peptides from single proteins modified by HOCl (100 and 500 μM, within estimated pathophysiological levels), at a high level of sensitivity and accuracy. Particular emphasis was placed on 1) sensitive and precise detection of modification sites, 2) the avoidance of loss or artefactual creation of modifications, 3) accurate quantification of peptide abundance and reduction of missing values problem, 4) monitoring the dynamics of modification in samples exposed to different oxidant concentrations and 5) development of guidelines for verification of chlorination sites assignment.
A combination of an optimised sample preparation protocol, and improved data analysis approaches have allowed identification of 33 and 15 chlorination sites in laminin and fibronectin, respectively, reported in previous manuscripts [1,2]. The method was subsequently tested on murine basement membrane extract, which contains high levels of laminin in a complex mixture. Here, 10 of the major chlorination sites in laminin were recapitulated, highlighting the utility of the method in detecting damage in complex samples.
Protein chlorination, Hypochlorous acid, Myeloperoxidase, Inflammation, Extracellular matrix proteins, Mass spectrometry, Proteomics Abbreviations: 3-ClTyr, 3-chlorotyrosine; 3,5-Cl2Tyr, 3,5-dichlorotyrosine; BME, basement membrane extracts; DTT, dithiothreitol; ECM, extracellular matrix; HCD, Higher energy collision dissociation; HOCl, the physiological mixture of hypochlorous acid and its anion -OCl; MS, mass spectrometry; RSO, relative site occupancy; SDC, sodium deoxycholate; TCA, trichloroacetic acid; TCEP, Tris(2-carboxyethyl)phosphine hydrochloride
Analysis of protein chlorination by mass spectrometry
Tina Nybo,a,b Michael J. Davies,a and Adelina Rogowska-Wrzesinskab,?
A cruciate ligament (CL) injury is a severe injury in soccer. Neuromuscular training programs have a well-documented preventive effect, but there are few studies on the effectiveness of such a program at a national level. The Swedish Knee Control Program (KCP) was found to be effective in preventing CL injuries in youth female soccer players. The KCP was implemented nationwide in Sweden in 2010.
To evaluate the effectiveness of the Swedish KCP in reducing acute knee injuries in soccer players at a nationwide level.
Descriptive epidemiology study.
All licensed soccer players in Sweden are covered by the same insurance company. Using this insurance database, around 17,500 acute knee injuries that were reported to the insurance company between 2006 and 2015 were included in the study. By matching the number of licensed soccer players with the number of reported injuries each year, the annual incidence of knee and CL injuries was able to be calculated. To evaluate the spread of the KCP nationally, a questionnaire was sent to all 24 Swedish district football associations (FAs) with questions regarding KCP education. The number of downloads of the KCP mobile application (app) was obtained.
The incidence of CL injuries decreased during the study period for both male (from 2.9 to 2.4 per 1000 player-years) and female players (from 4.9 to 3.9 per 1000 player-years). The overall incidence of knee injuries decreased in both male (from 5.6 to 4.6 per 1000 player-years) and female players (from 8.7 to 6.4 per 1000 player-years). Comparing before and after the nationwide implementation of the KCP, there was a decrease in the incidence of CL injuries by 6% (rate ratio [RR], 0.94 [95% CI, 0.89-0.98]) in male players and 13% (RR, 0.87 [95% CI, 0.81-0.92]) in female players and a decrease in the incidence of knee injuries by 8% (RR, 0.92 [95% CI, 0.89-0.96]) and 21% (RR, 0.79 [95% CI, 0.75-0.83]), respectively (P < .01 for all). This trend corresponded to a reduction of approximately 100 CL injuries each year in Sweden. A total of 21 of 24 district FAs held organized KCP educational courses during the study period. The percentage of district FAs holding KCP courses was between 46% and 79% each year. There were 101,236 downloads of the KCP app. Conclusion: The KCP can be considered partially implemented nationwide, and the incidence of knee and CL injuries has decreased in both sexes at a nationwide level.
injury prevention, cruciate ligament, coach education, insurance data, nationwide implementation
A Nationwide Follow-up Survey on the Effectiveness of an Implemented Neuromuscular Training Program to Reduce Acute Knee Injuries in Soccer Players
Malin aman, RPT, PhD,*† Karin Larsen, MD, Prof.,‡ Magnus Forssblad, MD, Assoc. Prof.,§ Annica Nasmark, RPT, MSc,∥ Markus Walden, MD, PhD,¶ and Martin Hagglund, RPT, Prof.¶
An aged circulatory environment can activate microglia, reduce neural precursor cell activity, and impair cognition in mice. We hypothesized that brain endothelial cells (BECs) mediate at least some of these effects. We observe BECs in the aged mouse hippocampus express an inflammatory transcriptional profile with focal upregulation of Vascular Cell Adhesion Molecule 1 (VCAM1), a protein that facilitates vascular-immune cell interactions. Concomitantly, the shed, soluble form of VCAM1 is prominently increased in plasma of aged humans and mice, and their plasma is sufficient to increase VCAM1 expression in cultured BECs and young mouse hippocampi. Systemic anti-VCAM1 antibody or genetic ablation of VCAM1 in BECs counteracts the detrimental effects of aged plasma on young brains and reverses aging aspects including microglial reactivity and cognitive deficits in old mouse brains. Together, these findings establish brain endothelial VCAM1 at the blood-brain barrier (BBB) as a possible target to treat age-related neurodegeneration.
Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1
Hanadie Yousef,1,2 Cathrin J. Czupalla,2,3,6 Davis Lee,1,2 Michelle B. Chen,4 Ashley N. Burke,1,2 Kristy A. Zera,1 Judith Zandstra,1,2 Elisabeth Berber,1,2 Benoit Lehallier,1,2 Vidhu Mathur,1,2 Ramesh V. Nair,5 Liana N. Bonanno,1,2 Andrew C. Yang,1,4 Todd Peterson,1 Husein Hadeiba,3,6 Taylor Merkel,1,2 Jakob Korbelin,7 Markus Schwaninger,8 Marion S. Buckwalter,1,9 Stephen R. Quake,4,10 Eugene C. Butcher,2,3,6 and Tony Wyss-Coray1,2,6,9
2019 Nov 13.