White crystalline powder
hydroxy-butanedioic acid/R,S(±)-Malic acid/Butanedioic acid, hydroxy-/alpha-Hydroxysuccinic acid/(±)-1-Hydroxy-1,2-ethanedicarboxylic Acid/Butanedioic acid, 2-hydroxy-/Hydroxybutandisaeure/alpha-hydroxysuccinicacid/MALIC ACID, (DL)/hydroxysuccinic acid/DL(+/-)-MALIC ACID/R,S-Malic acid/2-hydroxybutane-1,4-dioic acid/2-hydroxybutanedioic acid/2-Hydroxysuccinic Acid/a-Hydroxysuccinic Acid/Malic acid/Malic acid, dl-/DL-MALIC ACID/2-Hydroxy-succinic acid/DL-Hydroxybutanedioic acid
306.4±27.0 °C at 760 mmHg
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For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:6915-15-7) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
In the present investigation, crude glycerol derived from transesterification process was utilized to produce the commercially-valuable malic acid. A combined resistant on methanol and malic acid strain of Aspergillus niger MTCC 281 mutant was generated in solid medium containing methanol (1-5%) and malic acid (40-80 g/L) by the adaptation process for 22 weeks. The ability of induced Aspergillus niger MTCC 281 mutant to utilize crude glycerol and pure glycerol to produce malic acid was studied. The yield of malic acid was increased with 4.45 folds compared with that of parent strain from crude glycerol. The highest concentration of malic acid from crude glycerol by using beneficial mutant was found to be 77.38 ± 0.51 g/L after 192 h at 25 °C. This present study specified that crude glycerol by-product from biodiesel production could be used for producing high amount of malic acid without any pretreatment.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Aspergillus niger MTCC281; Biodiesel; Crude glycerol; Induced mutant strain; Malic acid
Malic acid production by chemically induced Aspergillus niger MTCC 281 mutant from crude glycerol.
Iyyappan J1, Bharathiraja B2, Baskar G3, Jayamuthunagai J4, Barathkumar S1, Anna Shiny R1.
In the present investigation, the effects of crude glycerol concentration, spore inoculum concentration, yeast extract concentration and shaking frequency on seed morphology of Aspergillus niger PJR1 on malic acid production were investigated and dispersed fungal mycelium with higher biomass (20.25 ± 0.91 g/L) was obtained when A. niger PJR1 grow on crude glycerol. Dry cell weight under dispersed fermentation was 21.28% higher than usual pellet fermentation. The optimal crude glycerol, nitrogen source and nitrogen source concentration were found to be 160 g/L, yeast extract and 1.5 g/L, respectively. Batch fermentation in a shake flask culture containing 160 g/L crude glycerol resulted in the yield of malic acid 83.23 ± 1.86 g/L, after 192 h at 25 °C. Results revealed that morphological control of A. niger is an efficient method for increased malic acid production when crude glycerol derived from biodiesel production is used as feedstock.
Copyright © 2018 Elsevier Ltd. All rights reserved.
Aspergillus niger; Batch fermentation; Crude glycerol; Malic acid
Malic acid production from biodiesel derived crude glycerol using morphologically controlled Aspergillus niger in batch fermentation.
Iyyappan J1, Baskar G2, Bharathiraja B1, Saravanathamizhan R3.
In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture concentration on separation of malic acid was investigated. Solvent system consisting of 2 M of trioctylamine in 1-octanol was found to be non-toxic to A. niger PJR1 and resulted in the maximum partition coefficient of 0.75 when the solvent mixture to liquid ratio of 1:2 used. A. niger PJR1 from crude glycerol using in situ reactive batch fermentation resulted in the maximum malic acid titer of 115.67 ± 3.5 g/L with the productivity of 0.53 g/L.h after 216 h. Further, fed batch extractive fermentation with crude glycerol resulted in malic acid titer of 131.48 ± 3.4 g/L with the productivity of 0.45 g/L.h after 288 h. Thus reactive extraction combined with in situ fermentation could become effective method for enhanced malic acid production.
Copyright © 2020 Elsevier Ltd. All rights reserved.
Aspergillus niger; In situ fermentation; Malic acid; Reactive extraction
Enhanced malic acid production using Aspergillus niger coupled with in situ product recovery.
Iyyappan J1, Baskar G2, Bharathiraja B3, Gopinath M1.
2020 Mar 27
Malic acid is a dicarboxylic acid that is naturally found in fruits such as apples and pears. It plays a role in many sour or tart foods.