This product is isolated and purified from the fruits of Siraitia grosvenorii Swingle.
Nat Prod Commun. 2009 Jun;4(6):769-72. A minor, sweet cucurbitane glycoside from Siraitia grosvenorii.[Pubmed: 19634319]A minor, sweet cucurbitane-glycoside, named iso-mogroside V (1), was isolated from Luo Han Guo (Siraitia grosvenorii (Swingle) C. Jeffery) along with five previously reported mogrosides. METHODS AND RESULTS: The structure of iso-mogroside V, 3-[(4-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]-mogrol-24-O-beta-D-glucopyranosyl-(1-->2)-O-[beta-D-glucopyranosyl-(1-->6)]beta-D-glucopyranoside, was established on the basis of extensive 2D-NMR (COSY, TOCSY, NOESY, HSQC, and HMBC) and LCMS analyses. CONCLUSIONS: The five known mogrosides were identified as mogroside V (2), 11-oxo-mogroside V (3), siamenoside I (4), Mogroside IVa (5) and Mogroside IVe (6). Iso-mogroside V was determined to be approximately 500 times sweeter than 0.5% (w/v) sucrose.
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
1177.2±65.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:88901-41-1) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Mogrosides, the main bioactive compounds isolated from the fruits of Siraitia grosvenorii, are a group of cucurbitane-type triterpenoid glycosides that exhibit a wide range of notable biological activities and are commercially available worldwide as natural sweeteners. However, the extraction cost is high due to their relatively low contents in plants. Therefore, molecular breeding needs to be achieved when conventional plant breeding can hardly improve the quality so far. In this study, the levels of 21 active mogrosides and two precursors in 15 S. grosvenorii varieties were determined by HPLC-MS/MS and GC-MS, respectively. The results showed that the variations in mogroside V content may be caused by the accumulation of cucurbitadienol. Furthermore, a total of four wild-type cucurbitadienol synthase protein variants (50R573L, 50C573L, 50R573Q, and 50C573Q) based on two missense mutation single nucleotide polymorphism (SNP) sites were discovered. An in vitro enzyme reaction analysis indicated that 50R573L had the highest activity, with a specific activity of 10.24 nmol min−1 mg−1. In addition, a site-directed mutant, namely, 50K573L, showed a 33% enhancement of catalytic efficiency compared to wild-type 50R573L. Our findings identify a novel cucurbitadienol synthase allele correlates with high catalytic efficiency. These results are valuable for the molecular breeding of luohanguo.
Siraitia grosvenorii, molecular breeding, site-directed mutant, mogrosides, cucurbitadienol synthase, single nucleotide polymorphism (SNP)
Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency
Jing Qiao,1 Zuliang Luo,1 Zhe Gu,1 Yanling Zhang,2 Xindan Zhang,2 and Xiaojun Ma1,*