Rumex japonicus/"Constit. of the heartwood of Maesopsis eminii, of the roots of Dianella laevis and Dianella nigra and of several Rumex spp."
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
413.2±40.0 °C at 760 mmHg
165-166℃ (methanol )
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:3785-24-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is well-known as the causal agent of Fusarium wilt of banana and is one of the most destructive phytopathogens for banana plants. The molecular mechanisms underlying Foc TR4 virulence remain elusive. Here, we demonstrate that a cerato-platanin (CP) protein, FocCP1, functions as a virulence factor that is required by Foc TR4 for penetration and full virulence. The FocCP1 gene was expressed in every condition studied, showing a high transcript level in planta at the early stage of infection. Infiltration of the recombinant FocCP1 protein induced significant cell death and upregulated defence-related gene expression. FocCP1 knock-out strains showed a significant decrease in aerial growth rather than aqueous growth, which is reminiscent of hydrophobins. Furthermore, deletion of FocCP1 significantly reduced virulence and dramatically reduced infective growth in banana roots, likely resulting from a defective penetration ability. Taken together, the results of this study provide novel insight into the function of the recently identified FocCP1 as a virulence factor in Foc TR4.
Fusarium oxysporum f. sp. cubense, cerato-platanin, virulence, necrosis
A Cerato-Platanin Family Protein FocCP1 Is Essential for the Penetration and Virulence of Fusarium oxysporum f. sp. cubense Tropical Race 4
Siwen Liu,1,2,† Bo Wu,2,† Jing Yang,2,† Fangcheng Bi,2 Tao Dong,2 Qiaosong Yang,2 Chunhua Hu,2 Dandan Xiang,2 Hongrui Chen,1 Huoqing Huang,2 Chuange Shao,2 Yixiang Chen,2 Ganjun Yi,2,* Chunyu Li,2,* and Xiuwu Guo1,*
The gene encoding N,N’-diacetylchitobiase (chitobiase) of the chitinolytic marine bacterium Vibrio harveyi has been isolated. While expression of the chitobiase gene (chb) was inducible by N,N’-diacetylchitobiose in V. harveyi, it was expressed constitutively when cloned in Escherichia coli, suggesting that controlling elements are not closely linked to chb. Chitobiase was found in the membrane fraction of E. coli cells containing plasmids with the cloned V. harveyi chb gene. When membranes of such cells were separated on Osborn gradients, chitobiase activity was found mainly in the outer membrane band. Translocation of the enzyme to the outer membrane was accompanied by cleavage of a signal peptide. A fusion protein, in which 22 amino acids from the amino terminus of prechitobiase were replaced with 21 amino acids from the pUC19 lacZ amino terminus, was not processed, and 99% of the activity was located in the cytoplasmic fraction. A homology to six amino acids surrounding the lipoprotein processing and modification site was found near the amino terminus of prechitobiase.
Translocation of Vibrio harveyi N,N'-diacetylchitobiase to the outer membrane of Escherichia coli.
M Jannatipour, R W Soto-Gil, L C Childers, and J W Zyskind
Synchronous cultures of the soil amoeba Acanthamoeba castellanii, established by a selection procedure, show significant oscillations of respiration and total cell protein. There was little difference between the period of these oscillations, which averaged 76 min, although the five incubation temperatures used varied between 20 degrees C and 30 degrees C and the cell division time increased from 7.8 to 16 hr. The phase of these oscillations also corresponded approximately at all incubation temperatures. Similar observations made over the whole division cycle at three temperatures indicated that similar oscillations occurred, with a constant period of 65 min, although these data were too variable to show this unequivocally. Control (asynchronous) cultures show that the oscillations are not a consequence of metabolic perturbation produced by the centrifugal selection procedure. It is suggested that these temperature-compensated epigenetic oscillations serve a dual role in cell cycle and circadian timekeeping and that cell cycle time is quantized.
Temperature-compensated oscillations in respiration and cellular protein content in synchronous cultures of Acanthamoeba castellanii.
D Lloyd, S W Edwards, and J C Fry