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Telocytes play key roles in maintenance of organ/tissue function and prevention of organ injury. However, there are great challenges to investigate telocytes functions using primary telocytes, due to the difficulties of isolation, identification, and stability. The present study aims at constructing continuous cell strain of mouse lung telocyte cell line with stable characters by gene modification and investigating biological behaviors and responses of gene-modified telocytes to inflammation.
Mouse primary lung telocytes were isolated and identified using immune-labeling markers and immunoelectron microscopy. Primary telocytes were transformed with Simian vacuolating virus 40 small and large T antigen (SV40). Biological characters, behaviors morphology, and proliferation of those gene-modified telocytes were defined and monitored dynamically for 50 generations, as compared with primary lung telocytes. Cell cycle of mouse primary lung telocytes or gene-modified telocytes was detected by flow cytometry.
Gene modified telocytes of generations 5, 10, 30 and 50 were observed with telopodes and also showed CD34 and ckit positive. Multiple cellular morphology were also observed on telocyte cell-line under monitor of celliq and enhanced cell proliferation were showed. SV40 transduction was also reduced apoptosis and increased the ratio of S and G2 phases in telocyte cell-line.
We successfully constructed mouse lung telocyte cell-line which maintained the biological properties and behaviors as primary telocytes and could responses to inflammation induced by LPS. Thus, gene-modified lung telocytes, Telocyte Line, would provide a cell tool for researchers exploring the roles and applications of telocytes involved in physiological and pathological states in future.
Electronic supplementary material
The online version of this article (10.1186/s12967-019-1870-y) contains supplementary material, which is available to authorized users.
Telocytes, Gene modification, Lung, SV40, Transformation
Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation
Dongli Song,#1 Menglin Xu,#1 Ruixue Qi,#1 Ruihua Ma,1 Yile Zhou,1 Duojiao Wu,corresponding author1,2 Hao Fang,corresponding author3,4 and Xiangdong Wangcorresponding author1,2
Although suicide could be an adverse health problem related to sensation seeking, this relationship has not been rigorously evaluated. The purpose of this study was to evaluate the relationship between sensation seeking and suicidality (suicidal ideation and plan) among adolescents and to test the influence of depressive symptom on this relationship.
We surveyed 2,017 adolescents in seven middle and high schools located in urban and rural areas in 2012. A self-report questionnaire included items about demographic characteristics, sensation seeking, depressive symptom, and suicide plans. We evaluated the influence of sensation seeking on suicide plan using multiple logistic regression and causal mediation analysis.
Sensation seeking was related to suicide ideation and plan. Sensation seeking was associated with a 13 % greater likelihood of a suicide plan during the past 12 months as the score increased by 1. After controlling for depressive symptom, the effect of sensation seeking was reduced, but still significantly increased the risk (adjusted odds ratio: 1.10; 95 % CI: 1.04-1.16). When depressive symptom was included as a potential mediator, depressive symptom exerted an indirect effect on suicide planning that constituted 37 % of the total effect of sensation seeking. There was no significant interaction between sensation seeking and either demographic variables or depressive symptom.
Sensation seeking can contribute to developing a suicide plan directly and indirectly via depressive symptom. Sensation seeking could be used to identify high-risk adolescents and provide proper interventions.
Electronic supplementary material
The online version of this article (doi:10.1186/s12889-016-2729-2) contains supplementary material, which is available to authorized users.
Sensation seeking, Adolescence, Suicide, Depression
Sensation seeking as a potential screening tool for suicidality in adolescence
Won Kyung Lee,1 Dohee Lim,2 Hye Ah Lee,2 and Hyesook Parkcorresponding author2