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Nystose

$152

  • Brand : BIOFRON

  • Catalogue Number : BD-P0761

  • Specification : 98.5%(HPLC&TLC)

  • CAS number : 13133-07-8

  • Formula : C24H42O21

  • Molecular Weight : 666.58

  • PUBCHEM ID : 166775

  • Volume : 25mg

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Catalogue Number

BD-P0761

Analysis Method

Specification

98.5%(HPLC&TLC)

Storage

-20℃

Molecular Weight

666.58

Appearance

Powder

Botanical Source

This product is isolated and purified from the roots of Morinda officinalis How.

Structure Type

Category

SMILES

C(C1C(C(C(C(O1)OC2(C(C(C(O2)CO)O)O)COC3(C(C(C(O3)CO)O)O)COC4(C(C(C(O4)CO)O)O)CO)O)O)O)O

Synonyms

α-D-Glucopyranoside, O-β-D-fructofuranosyl-(2->1)-O-β-D-fructofuranosyl-(2->1)-β-D-fructofuranosyl/β-D-Fructofuranosyl-(2->1)-β-D-fructofuranosyl-(2->1)-β-D-fructofuranosyl α-D-glucopyranoside/NISTOSE/Nystose Trihydrate/Fungitetraose/Fungitetraose Trihydrate/Nystose

IUPAC Name

Applications

Food Chem. 2015 Dec 1;188:658-63. Comparative study on the effects of nystose and fructofuranosyl nystose in the glycation reaction on the antigenicity and conformation of β-lactoglobulin.[Pubmed: 26041244]Our previous work indicated that the antigenicity of bovine β-lactoglobulin (β-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes.METHODS AND RESULTS: In attempt to unravel further changes of β-LG antigenicity, Nystose (GF3) and 1(F)-β-fructofuranosyl Nystose (GF4) of FOS were used to investigate the relationship between conformation and antigenicity. The antigenicity of β-LG after conjugated with GF3 and GF4 decreased from 143.4 to 29.5 and 31.6 μg/mL, respectively. The results of mass spectrometry revealed that the molecular weight of β-LG increased from 18.4 to 19.8 and 19.1 kDa after conjugation with GF3 and GF4, respectively.CONCLUSIONS: It was shown that the conformational changes of β-LG after conjugation with GF3 were bigger than that with GF4, including quenching of fluorescence intensity, the red-shift of fluorescence spectra, and the increase in sulfhydryl content. However, there was no significant difference in the antigenicity between β-LG-GF3 and β-LG-GF4 conjugates (P>0.05).

Density

1.84±0.1 g/cm3

Solubility

DMSO; Water

Flash Point

604.3±34.3 °C

Boiling Point

1075.7±65.0 °C at 760 mmHg

Melting Point

130-133 ºC

InChl

InChl Key

FLDFNEBHEXLZRX-DLQNOBSRSA-N

WGK Germany

RID/ADR

HS Code Reference

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:13133-07-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

No Technical Documents Available For This Product.

PMID

2139618

Abstract

1. The effect of nystose on cariogenic factors of mutans streptococci was investigated. 2. Nystose was not utilized as a substrate for the growth, lactic acid production, plaque formation and cellular aggregation of serotype a-g strains of mutans streptococci. 3. Furthermore, nystose did not served as a substrate for water-insoluble glucan formation by glucosyltransferase (GTase) from strains used. 4. In the cellular adherence experiment using nystose, the amount of serotype d cells that adhered to glass surface was almost zero. 5. In the presence of sucrose, nystose inhibited GTase activity and adherence of cells. 6. Finally, significantly lower caries score was observed in rats infected with Strep. sobrinus and fed with diet containing nystose as compared with sucrose.

Title

Low-cariogenicity of the Tetrasaccharide Nystose

Author

T Ikeda 1 , T Kurita, H Hidaka, S M Michalek, M Hirasawa

Publish date

1990

PMID

3295144

Abstract

Nystose represents a fructooligosaccharide with two fructose molecules linked via beta(1—-2) bonds to the fructosyl moiety of sucrose. This tetrasaccharide was subjected to an array of in vitro tests designed for the assessment of potential sugar substitutes before animal or human studies. beta-Fructosidase from yeast cleaved nystose at about 5% of the initial rate observed with sucrose. The terminal fructose was released first. Glycosyltransferase from Streptococcus mutans #620 did not utilize nystose for the formation of a glucan-type polysaccharide. Anaerobic fermentation of nystose by a suspension of mixed dental plaque microorganisms and by S. mutans NCTC 10449 was about half as fast as with sucrose. Thin-layer chromatography at various reaction times with S. mutans NCTC 10449 indicated the terminal fructose as the site of first attack. Analyses for free monosaccharides confirmed these data because free fructose exceeded free glucose at early reaction times far more than would follow from the 3:1 ratio of fructose to glucose in the nystose molecule. High pressure liquid chromatography assays demonstrated lactic and acetic acids as the main fermentation products. Carbohydrases from human jejunal mucosa did not attack nystose. However, cecal anaerobic microorganisms of the rat fermented nystose rapidly into acids.

Title

In Vitro Assessment of Nystose as a Sugar Substitute

Author

S C Ziesenitz, G Siebert

Publish date

1987 May

PMID

26041244

Abstract

Our previous work indicated that the antigenicity of bovine β-lactoglobulin (β-LG) decreased after conjugation with fructo-oligosaccharides (FOS) which was related to its conformational changes. In attempt to unravel further changes of β-LG antigenicity, nystose (GF3) and 1(F)-β-fructofuranosyl nystose (GF4) of FOS were used to investigate the relationship between conformation and antigenicity. The antigenicity of β-LG after conjugated with GF3 and GF4 decreased from 143.4 to 29.5 and 31.6 μg/mL, respectively. The results of mass spectrometry revealed that the molecular weight of β-LG increased from 18.4 to 19.8 and 19.1 kDa after conjugation with GF3 and GF4, respectively. It was shown that the conformational changes of β-LG after conjugation with GF3 were bigger than that with GF4, including quenching of fluorescence intensity, the red-shift of fluorescence spectra, and the increase in sulfhydryl content. However, there was no significant difference in the antigenicity between β-LG-GF3 and β-LG-GF4 conjugates (P>0.05).

KEYWORDS

Antigenicity; Conformation; Conjugation; β-Lactoglobulin.

Title

Comparative Study on the Effects of Nystose and Fructofuranosyl Nystose in the Glycation Reaction on the Antigenicity and Conformation of β-Lactoglobulin

Author

Junzhen Zhong 1 , Yue Tu 1 , Wei Liu 1 , Shunjing Luo 2 , Chengmei Liu 3

Publish date

2015 Dec 1