Kemester 115/Kemester 104/methyl cis-9-octadecenoate/Kemester 105/Emery 2219/Methyl cis-9-octadecenoate C18:1/Methyl cis-9-octadecenoate,Oleic acid methyl ester/emerest2801/methyl 9-octadecenoate/Esterol 112/Methyl Oleate/Methyloleat/(Z)-9-Octadecenoic acid methyl ester/Emerest 2301/Oleic Acid Methyl Ester/Emery 2301
Methanol; Chloroform; DMSO
351.4±0.0 °C at 760 mmHg
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provides coniferyl ferulate(CAS#:112-62-9) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.
Bacillus licheniformis; lipase; oil cake industry waste; response surface methodology; solid state fermentation
Statistical optimization for lipase production from solid waste of vegetable oil industry.
Sahoo RK1, Kumar M2, Mohanty S3, Sawyer M4, Rahman PKSM4, Sukla LB5, Subudhi E1.
2018 Apr 21
In order to design proteins with improved properties i.e. thermostability, catalytic efficiency and to understand the mechanisms underlying, a thermostable variant of Bacillus lipase was generated by site-directed mutagenesis with enhanced thermal (∆Tm = + 12 °C) and chemical (∆Cm denaturation for Gdmcl = + 1.75 M) stability as compared to WT. Arg153-His variant showed 72-fold increase in thermostability (t 1/2 = 6 h) at 60 °C as compared to WT (t 1/2 = 5 min). Increase in thermostability might be contributed by the formation of additional hydrogen bonds between His153/AO-Arg106/ANH2 as well as His153-Arg106/ANE. The variant demonstrated broad substrate specificity. A maximum conversion of 59 and 62% was obtained for methyl oleate and methyl butyrate, respectively, using immobilized variant lipase, whereas immobilized WT enzyme synthesizes 35% methyl oleate. WT enzyme was unable to synthesize methyl butyrate as it showed negligible activity with pNP-butyrate.
Fluorescence; GdmCl unfolding; Immobilization; Lipase; Methyl ester; Thermostability
Point mutation Arg153-His at surface of Bacillus lipase contributing towards increased thermostability and ester synthesis: insight into molecular network.
Chopra N1, Kaur J2.
One approach to improve the oxidative stability of biodiesel is the partial hydrogenation of carbon-carbon double bonds. In the current work, an efficient catalytic system using Pd(OAc)2 dissolved in polyethylene glycol (PEG) which in situ generates palladium nanoparticles was developed in order to promote a selective partial hydrogenation reaction of sunflower oil FAMEs into mono-hydrogenated products avoiding the formation of saturated compounds or trans-isomers. High content of methyl oleate (85.0±1.4%) was obtained by hydrogenation of sunflower oil biodiesel with only 7.0±0.2% stearic acid. Through evaluating the palladium nanoparticles by TEM analysis, it is observed that 4 nm palladium nanoparticles generated in situ in PEG4000 are highly selective for the partial hydrogenation of sunflower oil biodiesel. And the Pd-PEG4000 catalyst can be resued for five times without obvious loss of activity or methyl oleate selectivity.
biodiesel; fatty acid methyl ester; hydrogenation; palladium nanoparticles; polyethylene glycol
Partial Hydrogenation of Sunflower Oil-derived FAMEs Catalyzed by the Efficient and Recyclable Palladium Nanoparticles in Polyethylene Glycol.
Liu W1, Lu G1.
2017 Oct 18