This product is isolated and purified from the herbs of Murraya exotica L.
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
418.8±45.0 °C at 760 mmHg
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Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:6545-99-9) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
The objective of this study was to develop a consecutive preparation method for the isolation and purification of hainanmurpanin, meranzin, and phebalosin from leaves of Murraya exotica L. The process involved supercritical fluid extraction with CO2 , solvent extraction, and two-step high-speed countercurrent chromatography. Pressure, temperature, and the volume of entrainer were optimized as 27 MPa, 52°C, and 60 mL by response surface methodology in supercritical fluid extraction with CO2 , and the yield of the crude extracts was 7.91 g from 100 g of leaves. Subsequently, 80% methanol/water was used to extract and condense the three compounds from the crude extracts, and 4.23 g of methanol/water extracts was obtained. Then, a two-step high-speed countercurrent chromatography procedure was developed for the isolation of the three target compounds from methanol/water extracts, including conventional high-speed countercurrent chromatography for further enrichment and consecutive high-speed countercurrent chromatography for purification. The yield of concentrates from high-speed countercurrent chromatography was 2.50 g from 4.23 g of methanol/water extracts. Finally, the consecutive high-speed countercurrent chromatography produced 103.2 mg of hainanmurpanin, 244.7 mg of meranzin, and 255.4 mg of phebalosin with purities up to 97.66, 99.36, and 98.64%, respectively, from 900 mg of high-speed countercurrent chromatography concentrates in one run of three consecutive sample loadings without exchanging a solvent system.
hainanmurpanin; high-speed countercurrent chromatography; meranzin; phebalosin; supercritical fluid extraction.
Preparative Isolation and Purification of Hainanmurpanin, Meranzin, and Phebalosin From Leaves of Murraya Exotica L. Using Supercritical Fluid Extraction Combined With Consecutive High-Speed Countercurrent Chromatography
Rongwei Yan 1, Jie Shen 1, Xiaojing Liu 2, Yong Zou 1 3, Xinjun Xu 1 3
Objective: To study the chemical constituents from ethyl acetate extract of Micromelum falcatum.
Methods: The constituents were separated and purified by silica gel, Sephadex LH-20 and HPLC. Their structures were elucidated by spectral analysis (NMR, MS).
Results: Ten compounds were isolated and identified as micropubescin (1), phebalosin (2), scopoletin (3), citrubuntin (4), thamnosmonin (5), hopeyhopin (6), arnottinin (7), casegravol (8), 2-methoxy-5-hydroxy cinnamate (9), threo-syringoylglycerol (10).
Conclusion: Compounds 1 – 10 are obtained from this plant for the first time.
[Study on the Chemical Constituents From Ethyl Acetate Extract of Micromelum Falcatum]
Sheng Huang 1, Xiong-Ming Luo, Jian-Hua Wang
Phebalosin From the Bark of Micromelum Minutum
V Tantishaiyakul, S Pummangura, C Chaichantipyuth, W W Ma, J L McLaughlin