This product is isolated and purified from the fruits of Forsythia suspensa
1,4-Cyclohexanediol, 1-(2-hydroxyethyl)-, cis-/Rengyol/cis-1-(2-Hydroxyethyl)-1,4-cyclohexanediol
Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
312.0±27.0 °C at 760 mmHg
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Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:93675-85-5) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Background. Recurrent Clostridium difficile infection (RCDI) is associated with repeated antibiotic treatment and the enhanced growth of antibiotic-resistant microbes. This study tested the hypothesis that patients with RCDI would harbor large numbers of antibiotic-resistant microbes and that fecal microbiota transplantation (FMT) would reduce the number of antibiotic-resistant genes.
Methods. In a single center study, patients with RCDI (n = 20) received FMT from universal donors via colonoscopy. Stool samples were collected from donors (n = 3) and patients prior to and following FMT. DNA was extracted and shotgun metagenomics performed. Results as well as assembled libraries from a healthy cohort (n = 87) obtained from the Human Microbiome Project were aligned against the NCBI bacterial taxonomy database and the Comprehensive Antibiotic Resistance Database. Results were corroborated through a DNA microarray containing 354 antibiotic resistance (ABR) genes.
Results. RCDI patients had a greater number and diversity of ABR genes compared with donors and healthy controls. Beta-lactam, multidrug efflux pumps, fluoroquinolone, and antibiotic inactivation ABR genes were increased in RCDI patients, although donors primarily had tetracycline resistance. RCDI patients were dominated by Proteobacteria with Escherichia coli and Klebsiella most prevalent. FMT resulted in a resolution of symptoms that correlated directly with a decreased number and diversity of ABR genes and increased Bacteroidetes and Firmicutes with reduced Proteobacteria. ABR gene profiles were maintained in recipients for up to a year following FMT.
Conclusions. RCDI patients have increased numbers of antibiotic-resistant organisms. FMT is effective in the eradication of pathogenic antibiotic-resistant organisms and elimination of ABR genes.
intestinal microbiome, antibiotics, colitis, antibiotic resistance, C. difficile
Fecal Microbial Transplants Reduce Antibiotic-resistant Genes in Patients With Recurrent Clostridium difficile Infection
Braden Millan,1,a Heekuk Park,1,a Naomi Hotte,1 Olivier Mathieu,2 Pierre Burguiere,2 Thomas A. Tompkins,2 Dina Kao,1,b and Karen L. Madsen1,b
2016 Jun 15;
Repetitive DNA sequences represent about half of the human genome. They have a central role in human biology, especially neurobiology, but are notoriously difficult to study. The purpose of this study was to quantify the transcription from repetitive sequences in a progerin-expressing cellular model of neuronal aging. Progerin is a nuclear protein causative of the Hutchinson-Gilford progeria syndrome that is also incrementally expressed during the normal aging process. A dedicated pipeline of analysis allowed to quantify transcripts containing repetitive sequences from RNAseq datasets oblivious of their genomic localization, tolerating a sufficient degree of mutational noise, all with low computational requirements. The pipeline has been applied to a published panel of RNAseq datasets derived from a well-established and well-described cellular model of aging of dopaminergic neurons. Progerin expression strongly downregulated the transcription from all the classes of repetitive sequences: satellites, long and short interspersed nuclear elements, human endogenous retroviruses, and DNA transposon. The Alu element represented by far the principal source of transcript originating either from repetitive sequences or from canonical coding genes; it was expressed on average at 192,493.5 reads per kilobase million (RPKM) (SE = 21,081.3) in the control neurons and dropped to 43,760.1 RPKM (SE = 5315.0) in the progerin-expressing neurons, being significant downregulated (p = 0.0005). The results highlighted a global perturbation of transcripts derived from repetitive sequences in a cellular model of aging and provided a direct link between progerin expression and alteration of transcription from human repetitive elements.
Electronic supplementary material
The online version of this article (10.1007/s11357-018-00050-2) contains supplementary material, which is available to authorized users.
Alu, Repetitive sequences, Progerin, Retrotransposon, Satellites
Progerin expression induces a significant downregulation of transcription from human repetitive sequences in iPSC-derived dopaminergic neurons
Walter Aranciocorresponding author
In the crystal of the title compound, C14H17NO2, molecules are arranged into chains along the b axis linked via O—H⋯O hydrogen bonds. While the benzene ring is essentially planar, with a maximum deviation from the best plane of 0.003 (1) a, the pyridine ring is slightly V-shaped: the distance of the carbonyl C atom from the benzene best plane is 0.120 (1) a. The hydroxy group is inclined markedly towards the benzene ring reducing the C—C—O bond angle to 113.21 (10)°.
Zuzana Kozubkova,a Marek Necas,b and Robert Vichaa,*
2010 Dec 1