Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
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provides coniferyl ferulate(CAS#:2170211-22-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
Leptin, a mediator of long-term regulation of energy balance, has been implicated in the release of adenohypophyseal gonadotropins by regulating gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. However, a direct effect of leptin on hormone release from gonadotropes remains virtually unexplored. In the current report, we assessed the long-term (48 h) actions of leptin on voltage-gated channel activity and luteinizing hormone (LH) production in mouse pituitary gonadotrope LβT2 cells. Electrophysiological recordings showed that leptin treatment significantly increased whole-cell patch clamp Ba2+ current through L-type Ca2+ channels. Quantitative RT-PCR analysis revealed increased levels of L-type (α1D) Ca2+ channel mRNA. Likewise, radioimmunoassays using specific antibodies provided evidence that leptin alone had no effect on LH release but did enhance GnRH-induced secretion of the hormone. Leptin had no apparent effects on LH gene transcription in absence of GnRH, as measured by transient transfection assays using a LH promoter-reporter gene and real time RT-PCR. These observations suggest that leptin might affect LH release by acting directly on the gonadotropes, favoring hormone production by enhancing responsiveness to GnRH as a result of increased Ca2+ channel expression.
Ca2+ channels, LβT2 cells, Leptin, Patch clamp, Pituitary, Gonadotropes
Leptin increases L-type Ca2+ channel expression and GnRH-stimulated LH release in LβT2 gonadotropes
Jose E. Avelino-Cruz,a,f Amira Flores,b Jorge Cebada,c Pamela L. Mellon,d Ricardo Felix,e and Eduardo Monjaraza,*
2010 Jan 27.