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provides coniferyl ferulate(CAS#:139-05-9) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
The interaction between sodium cyclamate (SC) and calf thymus DNA in simulated physiological buffer (pH 7.4) using ethidium bromide (EB) as fluorescence probe was investigated by UV-vis spectrometry (UV), fluorescence, resonance light scattering (RLS) and Fourier transform infrared (FT-IR) spectroscopy, along with DNA melting studies and cyclic voltammetric (CV) measurements. The results indicate that SC can not only bind into the minor groove of DNA, but also intercalate into the DNA Base pairs. Based on UV data, the binding constant K and binding sites n of the formed DNA/SC complex were estimated to be 2.83 × 103 mol/L and 2.0, respectively. Fluorescence results demonstrate that the quenching of DNA/EB induced by SC can mainly be attributed to static procedure. The melting studies and CV analysis further confirm that the interaction mechanism between the SC and DNA is similar to that of DNA intercalator.The results of FT-IR spectra show that a specific interaction mainly exist between SC and adenine and guanine bases of DNA, which resulting in potential damage due to some change in the information structure. The DNA saturation binding value estimated to be 1.67 based on the RLS data also indicated that SC may cause damage of DNA.
Copyright © 2019 Elsevier B.V. All rights reserved.
Cyclamate; DNA; Interaction; Spectroscopy
Interaction studies of sodium cyclamate with DNA revealed by spectroscopy methods.
Hu Y1, Xie M2, Wu X2.
2019 Sep 5
Screening for novel anticonvulsant drugs requires appropriate animal seizure models. Zebrafish provide small, accessible, and cost-efficient preclinical models applicable to high-throughput small molecule screening. Based on previous results in rodents, we have here examined the effects of artificial sweetener sodium cyclamate and antimicrobial agent sodium propylparaben on a model of pentylenetetrazole (PTZ)-induced seizures in zebrafish. Sodium cyclamate reduced the bursts of hyperactivity, the spasms, increased the latency to spasms, and the latency to seizure, while propylparaben increased the latency to spasms. The results show the potential of zebrafish to detect novel anticonvulsant compounds while they also demonstrate the ability of two commonly ingested chemical compounds to modify the seizure threshold when were administrated at low concentration.
© 2017 Wiley Periodicals, Inc.
animal behavior; anticonvulsant drugs; seizures; zebrafish
Anticonvulsant effect of sodium cyclamate and propylparaben on pentylenetetrazol-induced seizures in zebrafish.
Pisera-Fuster A1, Otero S1, Talevi A2, Bruno-Blanch L2, Bernabeu R1.
An accurate determination of quantitative and confirmative method for sodium cyclamate in liquor by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with linear trap technology has been established. Without pretreatment, the sample was directly injected after filtering through a 0.2 microm micro filter. The HPLC separation was performed on an Atlantis dC18 column (150 mm x 2.1 mm, 3 microm) by gradient elution with methanol and water containing 0.1% (v/v) formic acid. The eluent was determined and confirmed in multiple reaction monitoring-enhanced product ion (MRM-EPI) scan mode. The acquired data from MRM for the quantitative determination, and the product ion spectra were used for library search for qualitative confirmatory analysis. External standard was used for the quantitative determination of sodium cyclamate in liquor, and good linearity (r = 0.9991) was obtained over the range of 1.320 – 132.0 microg/L. The limit of detection (LOD, S/N = 3) for sodium cyclamate was 0.1 microg/L. The average recoveries ranged from 96.38% to 107.2% at the spiked levels of 2.640, 26.40 and 100.0 microg/L with the relative standard deviations (RSDs) less than 9%. The matching degrees of the spectra for all positive samples were higher than 92%. The method is simple, accurate and efficient for the determination of sodium cyclamate in liquor and particularly suitable for confirmatory analysis of positive samples.
[Determination of sodium cyclamate in liquor by high performance liquid chromatography-tandem mass spectrometry with linear trap technology].
Fang H1, Zhou Y, Lu Y, Jiang X, Yang Y.