White needle crystal
1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione/1-(2-Deoxy-β-D-erythro-pentofuranosyl)-4-hydroxy-5-methyl-2(1H)-pyrimidinone/Thymidine/1-(2-Deoxy-β-D-erythro-pentofuranosyl)-4-hydroxy-5-methylpyrimidin-2(1H)-one/2'-deoxy-5-methyluridine/2,4(1H,3H)-Pyrimidinedione, 1-(2-deoxy-.β.-D-erythro-pentofuranosyl)-5-methyl-/T6NVMVJ E1 A- ET5OTJ B1Q CQ &&Ribo Form/2,4(1H,3H)-Pyrimidinedione,1-(2-deoxy-.Beta.-D-erythro-pentofuranosyl)-5-methyl/SS-THYMIDINE/2,4(1H,3H)-Pyrimidinedione, 1-(2-deoxy-β-D-erythro-pentofuranosyl)-5-methyl-/Thyminedeoxyriboside/2'-dT/1-(2-Deoxy-b-D-ribofuranosyl)-5-methyluracil/Zidovudine Related Compound D/deoxyribosylthymine/deoxythymidine/DTHD/1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil/Thymine-2-deoxyriboside/1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-methylpyrimidin-2,4(1H,3H)-dion/2'-Deoxythymidine/Thymine deoxyriboside/2(1H)-pyrimidinone, 1-(2-deoxy-β-D-erythro-pentofuranosyl)-4-hydroxy-5-methyl-/3,4-Dihydrothymidine, 2'-deoxy-/2'-deoxy-5-methyl-Uridine/5-Methyldeoxyuridine/3H-Thymidine/1-(2-Deoxy-b-D-erythro-pentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione/Dthyd/1-(2-Deoxy-β-D-ribofuranosyl)thymine/5-methyl-2'-deoxyuridine/b-Thymidine/Thymidin
Methanol; Ethanol; Ethyl Acetate; Acetone; Pyridine; Water
510.1±60.0 °C at 760 mmHg
HS Code Reference
Personal Projective Equipment
For Reference Standard and R&D, Not for Human Use Directly.
provides coniferyl ferulate(CAS#:50-89-5) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate
The relaxation dynamics of thymine and its derivatives thymidine and thymidine monophosphate are studied using time-resolved photoelectron spectroscopy applied to a water microjet. Two absorption bands are studied; the first is a bright ππ* state which is populated using tunable-ultraviolet light in the range 4.74-5.17 eV and probed using a 6.20 eV probe pulse. By reversing the order of these pulses, a band containing multiple ππ* states is populated by the 6.20 eV pulse and the lower energy pulse serves as the probe. The lower lying ππ* state is found to decay in ∼400 fs in both thymine and thymidine independent of pump photon energy, while thymidine monophosphate decays vary from 670 to 840 fs with some pump energy dependence. The application of a computational quantum mechanical/molecular mechanical scheme at the XMS-CASPT2//CASSCF/AMBER level of theory suggests that conformational differences existing between thymidine and thymidine monophosphate in solution account for this difference. The higher lying ππ* band is found to decay in ∼600 fs in all three cases, but it is only able to be characterized when the 5.17 eV probe pulse is used. Notably, no long-lived signal from an nπ* state can be identified in either experiment on any of the three molecules.
Relaxation Dynamics of Hydrated Thymine, Thymidine, and Thymidine Monophosphate Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy.
Erickson BA1, Heim ZN1, Pieri E2, Liu E1, Martinez TJ2, Neumark DM1,3.
2019 Dec 19
Mitochondrial thymidine kinase 2 (TK2) catalyzes the phosphorylation of thymidine (dT) and deoxycytidine (dC) and is essential for mitochondrial function in post-mitotic tissues. The phosphorylation of dT shows negative cooperativity, but the phosphorylation of dC follows classical Michaelis-Menten kinetics. The enzyme is feedback-inhibited by its end products deoxythymidine triphosphate (dTTP) and deoxycytidine triphosphate (dCTP). In order to better understand the reaction mechanism and the negative cooperative behavior, we conducted isothermal titration calorimetry (ITC) and intrinsic tryptophan fluorescence (ITF) quenching studies with purified recombinant human TK2. Cooperative binding was observed with dT but not dC by the ITC analysis in accordance with earlier enzyme kinetic studies. The phosphate donor adenosine triphosphate (ATP) did not bind to either dTTP-bound or dTTP-free enzymes but bound tightly to the dT- or dC-TK2 complexes with large differences in enthalpy and entropy changes, strongly suggesting an ordered binding of the substrates and different conformational states of the ATP and dT- and dC-TK2 ternary complexes. dTTP binding was endothermic; however, dCTP could not be shown to interact with the enzyme. ITF quenching studies also revealed tight binding of dT, dC, deoxythymidine monophosphate, deoxycytidine monophosphate, and dTTP but not adenosine 5′-diphosphate or ATP. These results strongly indicate an ordered sequential binding of the substrates and ordered release of the products as well as different conformational states of the active site of TK2. These results help to explain the different kinetics observed with dT and dC as substrates, which have important implications for TK2 regulation in vivo.
Negative Cooperative Binding of Thymidine, Ordered Substrate Binding, and Product Release of Human Mitochondrial Thymidine Kinase 2 Explain Its Complex Kinetic Properties and Physiological Functions.
Wang L1, Zhang L2, Sun R3, Eriksson S1.
2018 Aug 13
When a neutral solution of thymidine and ascorbic acid was irradiated with UV light of wavelength longer than 300 nm in the presence of salicylic acid as a photosensitizer, six product peaks appeared in an HPLC chromatogram in addition to small amounts of thymidine dimers. The six products were identified as three pairs of diastereomers of 5-(2-deoxy-2-l-ascorbyl)-5,6-dihydrothymidine, 5-(2-l-ascorbyl)-5,6-dihydrothymidine, and 5,6-dihydrothymidine. These results suggest that novel DNA damage may be generated by ascorbic acid with salicylic acid induced by sunlight.
Copyright © 2019 Elsevier Ltd. All rights reserved.
Ascorbic acid; Salicylic acid; Thymidine; Ultraviolet light
Reaction of thymidine and ascorbic acid induced by UV in the presence of salicylic acid.
Suzuki T1, Kishida Y2.
2019 Oct 1