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Wilforlide A

$113

  • Brand : BIOFRON

  • Catalogue Number : BF-W2001

  • Specification : 98%

  • CAS number : 84104-71-2

  • Formula : C30H46O3

  • Molecular Weight : 454.68

  • PUBCHEM ID : 158477

  • Volume : 20mg

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Catalogue Number

BF-W2001

Analysis Method

HPLC,NMR,MS

Specification

98%

Storage

2-8°C

Molecular Weight

454.68

Appearance

White powder

Botanical Source

herb of Tripterygium wilfordii Hook.f.

Structure Type

Terpenoids

Category

Standards;Natural Pytochemical;API

SMILES

CC1(C2CCC3(C(C2(CCC1O)C)CC=C4C3(CCC5(C4CC6(CC5OC6=O)C)C)C)C)C

Synonyms

Olean-12-en-29-one, 22,29-epoxy-3-hydroxy-, (3β,22α)-/WOLFORLIDE A/(3β,22α)-3-Hydroxy-22,29-epoxyolean-12-en-29-one/Regelide/Wilforlide/WilforlideA/abruslactone A

IUPAC Name

(1S,2R,5S,6R,9R,11S,14R,15R,19S,21S)-11-hydroxy-2,5,6,10,10,14,21-heptamethyl-23-oxahexacyclo[19.2.1.02,19.05,18.06,15.09,14]tetracos-17-en-22-one

Density

1.1±0.1 g/cm3

Solubility

Flash Point

208.6±22.9 °C

Boiling Point

555.0±50.0 °C at 760 mmHg

Melting Point

InChl

InChl Key

WGK Germany

RID/ADR

HS Code Reference

2932990000

Personal Projective Equipment

Correct Usage

For Reference Standard and R&D, Not for Human Use Directly.

Meta Tag

provides coniferyl ferulate(CAS#:84104-71-2) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of coniferyl ferulate are included as well.>> amp version: coniferyl ferulate

PMID

29787994

Abstract

Wilforlide A (WA), an active compound in Tripterygium wilfordii Hook F (TW) which is a traditional Chinese medicine for treatment of autoimmune diseases, is a quality control marker for TW product. At present, the bioavailability/pharmacokinetics of WA is not known. Such information is not only essential to evaluate the relevance of WA as a quality control maker, but also important for future clinical efficacy studies. Therefore, a high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometric method (HPLC-APCI-MS/MS) was developed and applied to a bioavailability/pharmacokinetic study of WA. WA and celastrol (the internal standard, IS) were extracted by a liquid-liquid extraction method using methyl tert-butyl ether. Multiple reaction monitoring (MRM) scanning in positive ionization mode was used to monitor the transition of m/z 455.1 to 191.3 for WA and 451.3 to 201.2 for IS. This method was validated and applied to a pharmacokinetic study of WA in mice following intravenous administration (IV, 1.2 mg/kg), intraperitoneal injection (IP, 6 mg/kg) and oral administration (PO, 30 mg/kg). The lower limit of quantification (LLOQ) for WA was 10 ng/ml. The intra- and inter-day precision was found to be within 15.4% while the accuracy within 94.1-115.7% for all the quality control and LLOQ samples. The samples were stable under all the usual storage and experimental conditions. The terminal elimination half-lives were 14.7, 9.1 and 22.7 min following IV, IP and PO dosing, while the absolute bioavailability for IP and PO WA were 9.39% and 0.58% respectively. These results indicated that the HPLC-APCI-MS/MS assay was suitable for the pharmacokinetic study of WA. WA was found poorly absorbed when given orally and therefore it may not be a relevant marker for the oral TW products in the market.

Copyright © 2018 Elsevier B.V. All rights reserved.

KEYWORDS

Bioavailability; HPLC-MS/MS; Tripterygium wilfordii; Wilforlide A

Title

Bioavailability of wilforlide A in mice and its concentration determination using an HPLC-APCI-MS/MS method.

Author

Wang Z1, Yeung S2, Chen S3, Moatazedi Y3, Chow MSS2.

Publish date

2018 Jul 15

PMID

27141802

Abstract

OBJECTIVE:
To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and to verify the method.

METHODS:
After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl (100 mm x 2.1 mm, 5 µm) with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization (ESI⁺) in positive mode was elected and the data was collected using multiple-reaction monitoring (MRM).

RESULTS:
The linearity was good (r > 0.995 0) and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%.

CONCLUSION:
This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.

Title

[Determination of Triptolide and Wilforlide A in Biological Samples by LC-MS/MS].

Author

Zhai JX, Liu W.

Publish date

2015 Dec

PMID

21608234

Title

[Study of wilforlide on foot cells and renal tubular epithelial cells protection].

Author

Zhou D, Zhang L, Sun W.

Publish date

2011 Apr


Description :

Wilforlide A is a natural product, separated from the ethanolic extract of tripterygium wilfordii. IC50 value:Target:In vitro:In vivo: Carrageenan-induced rat pedal swelling, tampon-induced rat granulation, and mice ear inhibition rate of swelling trail results show that high-dose wilforlide A has obvious anti-inflammatory effect, but has no significant immune suppressive activity [1].